Co-culture of $\beta-cells$ and endothelial cells in a micropatterned collagen sheet = 미세패턴이 집적된 콜라겐 시트를 이용한 베타세포와 혈관내피세포의 공배양

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Co-culture of beta cells and endothelial cells in constructing a pancreatic pseudo-tissue is important in understanding cell to cell interaction in islets, as well as understanding pancreatic diseases including diabetes. In this thesis, we constructed a pancreatic model where MIN6 and MS-1 cells are co-cultured within collagen sheets. In 4 to 10 days, depending on the cell seeding concentration, MIN6 cells formed islet-like clusters surrounded by a MS-1 cell monolayer. The MS-1 cells also formed monolayers at the edge of the hexagonal micropattern, resulting in a blood vessel-like structure in which no cells were found. MS-1 intra-islet vessels were formed, but they had a larger diameter than the in vivo intra-islet capillaries. Along with the decreased MIN6 insulin secretion in the co-cultured sheet compared to the mono-cultured sheet, our co-culture model showed the potential as a pancreatic disease model in which a similar change in beta cells and endothelial cells was observed. In addition, by simply embedding the cell mixture and the hexagonal micropattern into the collagen sheet, we were able to achieve high throughput yield, high reproducibility, and a tissue-level fabrication of the construct.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2018.2,[iv, 45 p. :]

Keywords

Beta cells▼aCollagen sheet▼aPancreatic islets▼aPseudo-tissue construction▼aVascular network; 베타세포▼a콜라겐 시트▼a췌장도▼a모사조직형성▼a혈관 네트워크

URI
http://hdl.handle.net/10203/266158
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734182&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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