Paper-based microfluidic device for angle-based colorimetric detection of an acetylcholinesterase inhibitor = 아세틸콜린 분해효소 저해제의 각도기반 비색검출을 위한 종이기반 미세유체장치 개발

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Due to the severe lethal effect of organophosphate (OP) compounds such as nerve agents, which irreversibly inhibit the activity of acetylcholinesterase (AChE), rapid on-site detection is essential. In this thesis, we developed a foldable paper-based analytical device (PAD) that was able to detect an AChE inhibitor in a semi-quantitative manner with simple procedures in the field. An angle-based readout of the yellow area in the paper channel was used for the semi-quantitative analysis with the naked eye. The yellow area on the paper channel was produced by the reaction of H2O2 from the enzymatic reaction and cerium oxide nanoparticles (nanoceria) immobilized on the ring-type paper channel. All reagents were pre-coated on the pop-up card shaped PAD to minimize the manual steps for dealing with multiple reagents such as sample mixing and sequential enzyme reactions. After confirming the optimal conditions, we could semi-quantitatively analyze a nerve agent simulant named dimethyl-methylphosphonate (DMMP) as an AChE inhibitor, by simply reading the angle of the yellow area in the paper channel. The foldable paper-based device proposed here would serve as a valuable tool that allows unskilled people to semi-quantitatively analyze AChE inhibitors, including nerve agents and OP pesticides.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2018.2,[iv, 45 p. :]

Keywords

Acetylcholinesterase inhibitor▼aAngle-based readout▼aCerium oxide nanoparticles▼aEnzyme inhibition assay▼aPaper-based analytical device; 각도기반 해석▼a산화세륨 나노입자▼a아세틸콜린 분해효소 저해제▼a종이기반 분석장치; 효소저해분석

URI
http://hdl.handle.net/10203/266156
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=734184&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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