DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jae Seong | ko |
dc.contributor.author | Kildegaard, Helene Faustrup | ko |
dc.contributor.author | Lewis, Nathan E. | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2019-09-03T01:20:05Z | - |
dc.date.available | 2019-09-03T01:20:05Z | - |
dc.date.created | 2019-06-10 | - |
dc.date.created | 2019-06-10 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | TRENDS IN BIOTECHNOLOGY, v.37, no.9, pp.931 - 942 | - |
dc.identifier.issn | 0167-7799 | - |
dc.identifier.uri | http://hdl.handle.net/10203/266086 | - |
dc.description.abstract | Mammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a long time. Advances in mammalian genome-editing technologies and systems biotechnology have shed light on clonal variation and enabled rational cell engineering in a targeted manner. We propose a new approach for a next-generation cell line development platform that can minimize clonal variation. Combined with the knowledge-based selection of ideal integration sites and engineering targets,targeted integration-based cell line development will allow tailored control of recombinant gene expression with predicted phenotypes. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE LONDON | - |
dc.title | Mitigating Clonal Variation in Recombinant Mammalian Cell Lines | - |
dc.type | Article | - |
dc.identifier.wosid | 000481640500006 | - |
dc.identifier.scopusid | 2-s2.0-85063035419 | - |
dc.type.rims | ART | - |
dc.citation.volume | 37 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 931 | - |
dc.citation.endingpage | 942 | - |
dc.citation.publicationname | TRENDS IN BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.tibtech.2019.02.007 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Lee, Jae Seong | - |
dc.contributor.nonIdAuthor | Kildegaard, Helene Faustrup | - |
dc.contributor.nonIdAuthor | Lewis, Nathan E. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Review | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | PROTEIN-PRODUCTION | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | SYSTEMS BIOTECHNOLOGY | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | HETEROGENEITY | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | SEQUENCE | - |
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