Real-time FLIM implementation by analog mean-delay method = 아날로그 평균 지연값 방식을 통한 실시간 형광 수명 현미경 구현

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Although analog mean-delay (AMD) method was introduced to enhance the imaging speed of commonly used fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting (TCSPC), a real-time image reconstruction using AMD method has not been implemented due to its data processing obsta-cles. In this paper, a real-time image restoration of AMD-FLIM is introduced through fast parallel data pro-cessing by using Threading Building Block (TBB, Intel) and octa-core processor (i7-5960x, Intel). Frame rate of 3.8 frames per second was achieved in 1024x1024 resolution with over 4 million lifetime determinations per second and measurement error within 10%. This image acquisition speed is 184 times faster than that of single-channel TCSPC and 9.2 times faster than that of 8-channel TCSPC (state-of-art photon counting rate of 80 million counts per second) with the same lifetime accuracy of 10% and the same pixel resolution.
Advisors
Gweon, Dae-Gabresearcher권대갑researcher
Description
한국과학기술원 :기계공학과,
Publisher
한국과학기술원
Issue Date
2016
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 기계공학과, 2016.2,[ix, 24 p. :]

Keywords

AMD FLIM▼afluorescence lifetime▼areal time; 형광 수명 현미경▼a실시간▼a아날로그 평균 지연값▼a형광 수명▼a병렬 처리

URI
http://hdl.handle.net/10203/265800
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=849863&flag=dissertation
Appears in Collection
ME-Theses_Master(석사논문)
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