DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Choi, Giltsu | - |
dc.contributor.advisor | 최길주 | - |
dc.contributor.author | Yoo, Yong Cheol | - |
dc.date.accessioned | 2019-08-22T02:44:26Z | - |
dc.date.available | 2019-08-22T02:44:26Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=849268&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/264823 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2015.2,[vi, 132 p. :] | - |
dc.description.abstract | Microglial activation in the central nervous system is a key event in the neuroinflammation that accompanies neurodegenerative diseases such as Alzheimer`s disease (AD). Among cytokines involved in microglial activation, amyloid $\beta$ (A$\beta$) peptide is known to be a key molecule in the induction of diverse inflammatory products, which may lead to chronic inflammation in AD. However, proteomic studies of microglia in AD are limited due to lack of proper cell or animal model systems. In this study, we performed a proteomic analysis of A$\beta$-stimulated human microglial cells using SILAC (stable isotope labeling with amino acids in cell culture) combined with LC-MS/MS. Results showed that 60 proteins increased or decreased their abundance by 1.5 fold or greater. Among these, ER-resident proteins such as SERPINH1, PDIA6, PDIA3, and PPIB were revealed to be key molecular biomarkers of human microglial activation, by validation of the proteomic results by immunostaining, PCR, ELISA, and western blot. Taken together, our data suggests that ER proteins play an essential role in human microglial activation by Aβ and may be important molecular therapeutic targets for treatment of AD. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | amyloid $\beta$▼ahuman microglia▼atandem mass tags▼aLC-MS/MS | - |
dc.subject | 아밀로이드 베타▼a인간 미세아교 세포▼a탠덤 매스 텍▼aLC-MS/MS | - |
dc.title | Proteomic analysis of human microglial cells activated by amyloid $\beta$ peptide | - |
dc.title.alternative | 아밀로이드 베타에 의해 활성화 된 인간 미세아교 세포의 프로테옴 분석 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.contributor.alternativeauthor | 유용철 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.