Reconstruction and optimization of the biosynthetic pathway for the mass production of minor ginsenosides마이너 진세노사이드 대량생산을 위한 대사회로 재설계 및 최적화 연구

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Ginseng is a medicinal herb that requires cultivation under shade conditions, typically for 6 yr, before harvesting. The principal components of ginseng are ginsenosides, glycosylated tetracyclic terpenes. Ginseno-sides are classified into two groups, protopanaxadiol (PPD) and protopanaxatriol (PPT), based on their hy-droxylation patterns, and further diverge to diverse ginsenosides through differential glycosylation. Three early enzymes, Dammarenediol-II synthase (PgDS) and two P450 enzymes, protopanaxadiol synthase (PgPPDS) and protopanaxatriol synthase (PgPPTS), have been reported, but glycosyltransferases that are necessary to synthesize specific ginsenosides have not yet been fully identified. To discover glycosyltransferases responsible for ginsenoside biosynthesis, we sequenced and assembled Panax ginseng transcriptome de novo and characterized four UDP-glycosyltransferases (PgUGTs): PgUGT74AE2, PgUGT94Q2, PgUGT71A27 and GpUGT23. PgUGT74AE2 transfers a glucose moiety from UDP-glucose (UDP-Glc) to the C3 hydroxyl groups of PPD and compound K to form Rh2 and F2, respectively, whereas PgUGT94Q2 transfers a glucose moiety from UDP-Glc to Rh2 and F2 to form Rg3 and Rd, respectively. PgUGT71A27 introduced glucose moiety to C20 hydroxyl group of PPD, Rh2, Rg3 and PPT and produced CK, F2, Rd and F1, respectively. GpUGT23 introduced glucose moiety to C20 position glucose of CK, F2, Rd and F1 and produced Gypenoside XLLV, gypenoside XVII, ginsenoside Rd and notoginsenoside U, respectively. Introduction of the combination of four UGTs into yeast together with PgDS and PgPPDS resulted in the de novo production of Rg3, F2, F1, Rd, CK and gypenoside XVII. Our results indicate that these four UGTs are key enzymes for the synthesis of ginsenosides and provide a method for producing specific ginsenosides through yeast fermentation
Advisors
Kim, Sun Changresearcher김선창researcher
Description
한국과학기술원 :생명과학과,
Publisher
한국과학기술원
Issue Date
2015
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2015.2,[vi, 96 p. :]

Keywords

minor ginsenosides▼aUDP-glycosyltrasferases▼aheterologous system▼aginsenoside biosynthesis▼apanax ginseng; 마이너 진세노사이드▼aUDP-당전이효소▼a진세노사이드 생합성▼a인삼

URI
http://hdl.handle.net/10203/264763
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=849269&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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