Active feedback control of holographic optical tweezers with optical diffraction tomography measurements광 회절 단층촬영법을 이용한 홀로그래픽 광학 집게의 동적 조작 기술 개발과 응용

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Optical tweezers have been an invaluable tool for manipulating 3-D positions of microscopic particles including colloidal and biological samples. In optical tweezers, a tightly focused laser beam generates a gradient force near the optical focus which can attract dielectric particles. So far, most optical trapping experiments have utilized spherical particles from the simple prediction of optical forces and the responding motion of samples. In contrast, the control of the 3-D behaviour of non-spherical particles with arbitrary orientations is highly complicated due to extensive computation and experimental difficulties. In this thesis, we achieved the real-time optical control of arbitrarily shaped particles by combining the wavefront shaping of a trapping beam and measurements of the 3-D refractive index (RI) distribution of samples. The thesis consists of two parts: (1) the development of holographic optical tweezers for engineering the 3-D light field distribution of a trapping beam, and (2) the development of real-time optical diffraction tomography (ODT) for measuring 3-D RI distribution of samples. In the first part, we investigate the principles of optical trapping force in various regimes depending on the size of samples, and demonstrate that trapping samples with the 3-D light field which shape resembles the 3-D geometry of the samples can provide stable orientation control of the arbitrarily shaped samples. In the second part, we investigate the principles and practical issues for utilizing ODT and develop the real-time ODT technique, which can measure the 3-D RI distribution of arbitrarily shaped samples in real-time. The real-time ODT technique is applied for measuring the 3-D RI distribution of various samples including colloidal PMMA dimers and red blood cells in different pathophysiological conditions, from which quantitative biochemical and morphological parameters are provided. Finally, we combine holographic optical tweezers and real-time ODT technique to realize a tomographic mould for optical trapping (TOMOTRAP) technique. In TOMOTRAP, engineering the 3-D intensity distribution of a trapping beam based on measured 3-D sample geometry generates a light mould enabling colloidal and biological samples to be aligned with arbitrary orientations and/or shapes. The present technique in this thesis provides stable orientation control and assembly of arbitrarily shaped particles without knowing a priori information about the sample geometry, which can offer direct applications in biophotonics and soft matter physics.
Advisors
Park, YongKeunresearcher박용근researcher
Description
한국과학기술원 :물리학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 물리학과, 2017.2,[vi, 95 p. :]

Keywords

holographic optical tweezers▼aoptical diffraction tomography▼aoptical trapping▼aholography▼awavefront shaping; 홀로그래픽 광학 집게▼a광 회절 단층촬영법▼a광 포획▼a홀로그래피▼a파면 제어

URI
http://hdl.handle.net/10203/264648
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=866909&flag=dissertation
Appears in Collection
PH-Theses_Ph.D.(박사논문)
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