Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems

Cited 321 time in webofscience Cited 265 time in scopus
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dc.contributor.authorMurray, Evanko
dc.contributor.authorCho, Jae Hunko
dc.contributor.authorGoodwin, Danielko
dc.contributor.authorKu, Taeyunko
dc.contributor.authorSwaney, Justinko
dc.contributor.authorKim, Sung-Yonko
dc.contributor.authorChoi, Heejinko
dc.contributor.authorPark, Young-Gyunko
dc.contributor.authorPark, Jeong-Yoonko
dc.contributor.authorHubbert, Austinko
dc.contributor.authorMcCue, Margaretko
dc.contributor.authorVassallo, Sarako
dc.contributor.authorBakh, Naveedko
dc.contributor.authorFrosch, Matthew P.ko
dc.contributor.authorWedeen, Van J.ko
dc.contributor.authorSeung, H. Sebastianko
dc.contributor.authorChung, Kwanghunko
dc.date.accessioned2019-08-06T03:20:08Z-
dc.date.available2019-08-06T03:20:08Z-
dc.date.created2019-08-06-
dc.date.created2019-08-06-
dc.date.created2019-08-06-
dc.date.created2019-08-06-
dc.date.created2019-08-06-
dc.date.issued2015-12-
dc.identifier.citationCELL, v.163, no.6, pp.1500 - 1514-
dc.identifier.issn0092-8674-
dc.identifier.urihttp://hdl.handle.net/10203/264051-
dc.description.abstractCombined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional phenotyping of both animal tissues and human clinical samples. This method, termed SWITCH, uniformly secures tissue architecture, native biomolecules, and antigenicity across an entire system by synchronizing the tissue preservation reaction. The heat-and chemical-resistant nature of the resulting framework permits multiple rounds (>20) of relabeling. We have performed 22 rounds of labeling of a single tissue with precise co-registration of multiple datasets. Furthermore, SWITCH synchronizes labeling reactions to improve probe penetration depth and uniformity of staining. With SWITCH, we performed combinatorial protein expression profiling of the human cortex and also interrogated the geometric structure of the fiber pathways in mouse brains. Such integrated high-dimensional information may accelerate our understanding of biological systems at multiple levels.-
dc.languageEnglish-
dc.publisherCELL PRESS-
dc.titleSimple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems-
dc.typeArticle-
dc.identifier.wosid000366044800022-
dc.identifier.scopusid2-s2.0-84949255266-
dc.type.rimsART-
dc.citation.volume163-
dc.citation.issue6-
dc.citation.beginningpage1500-
dc.citation.endingpage1514-
dc.citation.publicationnameCELL-
dc.identifier.doi10.1016/j.cell.2015.11.025-
dc.contributor.localauthorKu, Taeyun-
dc.contributor.localauthorPark, Young-Gyun-
dc.contributor.nonIdAuthorMurray, Evan-
dc.contributor.nonIdAuthorCho, Jae Hun-
dc.contributor.nonIdAuthorGoodwin, Daniel-
dc.contributor.nonIdAuthorSwaney, Justin-
dc.contributor.nonIdAuthorKim, Sung-Yon-
dc.contributor.nonIdAuthorChoi, Heejin-
dc.contributor.nonIdAuthorPark, Jeong-Yoon-
dc.contributor.nonIdAuthorHubbert, Austin-
dc.contributor.nonIdAuthorMcCue, Margaret-
dc.contributor.nonIdAuthorVassallo, Sara-
dc.contributor.nonIdAuthorBakh, Naveed-
dc.contributor.nonIdAuthorFrosch, Matthew P.-
dc.contributor.nonIdAuthorWedeen, Van J.-
dc.contributor.nonIdAuthorSeung, H. Sebastian-
dc.contributor.nonIdAuthorChung, Kwanghun-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusGLUTARALDEHYDE FIXATION-
dc.subject.keywordPlusPYRAMIDAL NEURONS-
dc.subject.keywordPlusCELL-MEMBRANE-
dc.subject.keywordPlusBRAIN-
dc.subject.keywordPlusCALRETININ-
dc.subject.keywordPlusFORMALDEHYDE-
dc.subject.keywordPlusPARVALBUMIN-
dc.subject.keywordPlusRESOLUTION-
dc.subject.keywordPlusCALBINDIN-
dc.subject.keywordPlusTISSUES-
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