DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ku, Taeyun | ko |
dc.contributor.author | Swaney, Justin | ko |
dc.contributor.author | Park, Jeong-Yoon | ko |
dc.contributor.author | Albanese, Alexandre | ko |
dc.contributor.author | Murray, Evan | ko |
dc.contributor.author | Cho, Jae Hun | ko |
dc.contributor.author | Park, Young-Gyun | ko |
dc.contributor.author | Mangena, Vamsi | ko |
dc.contributor.author | Chen, Jiapei | ko |
dc.contributor.author | Chung, Kwanghun | ko |
dc.date.accessioned | 2019-08-06T03:20:06Z | - |
dc.date.available | 2019-08-06T03:20:06Z | - |
dc.date.created | 2019-08-06 | - |
dc.date.created | 2019-08-06 | - |
dc.date.created | 2019-08-06 | - |
dc.date.created | 2019-08-06 | - |
dc.date.created | 2019-08-06 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | NATURE BIOTECHNOLOGY, v.34, no.9, pp.973 - + | - |
dc.identifier.issn | 1087-0156 | - |
dc.identifier.uri | http://hdl.handle.net/10203/264050 | - |
dc.description.abstract | The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnified analysis of the proteome (MAP), linearly expands entire organs fourfold while preserving their overall architecture and three-dimensional proteome organization. MAP is based on the observation that preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization allows for natural expansion upon protein denaturation and dissociation. The expanded tissue preserves its protein content, its fine subcellular details, and its organ-scale intercellular connectivity. We use off-the-shelf antibodies for multiple rounds of immunolabeling and imaging of a tissue's magnified proteome, and our experiments demonstrate a success rate of 82% (100/122 antibodies tested). We show that specimen size can be reversibly modulated to image both inter-regional connections and fine synaptic architectures in the mouse brain. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues | - |
dc.type | Article | - |
dc.identifier.wosid | 000383348500024 | - |
dc.identifier.scopusid | 2-s2.0-84986893145 | - |
dc.type.rims | ART | - |
dc.citation.volume | 34 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 973 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | NATURE BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1038/nbt.3641 | - |
dc.contributor.localauthor | Ku, Taeyun | - |
dc.contributor.localauthor | Park, Young-Gyun | - |
dc.contributor.nonIdAuthor | Swaney, Justin | - |
dc.contributor.nonIdAuthor | Park, Jeong-Yoon | - |
dc.contributor.nonIdAuthor | Albanese, Alexandre | - |
dc.contributor.nonIdAuthor | Murray, Evan | - |
dc.contributor.nonIdAuthor | Cho, Jae Hun | - |
dc.contributor.nonIdAuthor | Mangena, Vamsi | - |
dc.contributor.nonIdAuthor | Chen, Jiapei | - |
dc.contributor.nonIdAuthor | Chung, Kwanghun | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MOUSE-BRAIN | - |
dc.subject.keywordPlus | ARRAY TOMOGRAPHY | - |
dc.subject.keywordPlus | NEURAL CIRCUITS | - |
dc.subject.keywordPlus | NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.subject.keywordPlus | RECONSTRUCTION | - |
dc.subject.keywordPlus | CONNECTOME | - |
dc.subject.keywordPlus | SYNAPSES | - |
dc.subject.keywordPlus | CLARITY | - |
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