DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jihong | ko |
dc.contributor.author | Choi, Dong-Wook | ko |
dc.contributor.author | Park, Chankyu | ko |
dc.contributor.author | Ryu, Kyoung-Seok | ko |
dc.date.accessioned | 2019-04-30T05:32:04Z | - |
dc.date.available | 2019-04-30T05:32:04Z | - |
dc.date.created | 2019-04-30 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY, v.19, no.3, pp.112 - 118 | - |
dc.identifier.issn | 1226-6531 | - |
dc.identifier.uri | http://hdl.handle.net/10203/261662 | - |
dc.description.abstract | Hsp31 protein is one of the members of DJ-1 superfamily proteins and has a dimeric structure of which molecular weight (MW) is 62 kDa. The mutation of DJ-1 is closely related to early onset of Parkinson's disease. Hsp31 displays Zn+2-binding activity and was first reported to be a holding chaperone in E. coli. Its additional glyoxalase III active has recently been characterized. Moreover, an incubation at 60 degrees C induces Hsp31 protein to form a high MW oligomer (HMW) in vitro, which accomplishes an elevated holding chaperone activity. The NMR technique is elegant method to probe any local or global structural change of a protein in responses to environmental stresses (heat, pH, and metal). Although the presence of the backbone chemical shifts (bbCSs) is a prerequisite for detailed NMR analyses of the structural changes, general HSQC-based triple resonance experiments could not be used for 62 kDa Hsp31 protein. Here, we prepared the per-deuterated Hsp31 and performed the TROSY-based triple resonance experiments for the bbCSs assignment. Here, detailed processes of per-deuteration and the NMR experiments are described for other similar NMR approaches. | - |
dc.language | English | - |
dc.publisher | KOREAN MAGNETIC RESONANCE SOC | - |
dc.title | Per-deuteration and NMR experiments for the backbone assignment of 62 kDa protein, Hsp31 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 19 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 112 | - |
dc.citation.endingpage | 118 | - |
dc.citation.publicationname | JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY | - |
dc.identifier.doi | 10.6564/JKMRS.2015.19.3.112 | - |
dc.identifier.kciid | ART002056786 | - |
dc.contributor.localauthor | Park, Chankyu | - |
dc.contributor.nonIdAuthor | Ryu, Kyoung-Seok | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Backbone chemical shift assignment | - |
dc.subject.keywordAuthor | Hsp31 | - |
dc.subject.keywordAuthor | NMR | - |
dc.subject.keywordAuthor | Per-deuteration | - |
dc.subject.keywordAuthor | TROSY | - |
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