DC Field | Value | Language |
---|---|---|
dc.contributor.author | Padavannil, Abhilash | ko |
dc.contributor.author | Sarkar, Prithwijit | ko |
dc.contributor.author | Kim, Seung Joong | ko |
dc.contributor.author | Cagatay, Tolga | ko |
dc.contributor.author | Jiou, Jenny | ko |
dc.contributor.author | Brautigam, Chad A. | ko |
dc.contributor.author | Tomchick, Diana R. | ko |
dc.contributor.author | Sali, Andrej | ko |
dc.contributor.author | D'Arcy, Sheena | ko |
dc.contributor.author | Chook, Yuh Min | ko |
dc.date.accessioned | 2019-04-24T13:14:03Z | - |
dc.date.available | 2019-04-24T13:14:03Z | - |
dc.date.created | 2019-04-22 | - |
dc.date.created | 2019-04-22 | - |
dc.date.created | 2019-04-22 | - |
dc.date.created | 2019-04-22 | - |
dc.date.created | 2019-04-22 | - |
dc.date.created | 2019-04-22 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | ELIFE, v.8, pp.e43630 | - |
dc.identifier.issn | 2050-084X | - |
dc.identifier.uri | http://hdl.handle.net/10203/261493 | - |
dc.description.abstract | We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9 center dot H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9 center dot H2A-H2B but assembles into a RanGTP center dot Importin-9 center dot H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome. | - |
dc.language | English | - |
dc.publisher | ELIFE SCIENCES PUBLICATIONS LTD | - |
dc.title | Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chperone | - |
dc.type | Article | - |
dc.identifier.wosid | 000463860400001 | - |
dc.identifier.scopusid | 2-s2.0-85064500313 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.beginningpage | e43630 | - |
dc.citation.publicationname | ELIFE | - |
dc.identifier.doi | 10.7554/eLife.43630 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Seung Joong | - |
dc.contributor.nonIdAuthor | Padavannil, Abhilash | - |
dc.contributor.nonIdAuthor | Sarkar, Prithwijit | - |
dc.contributor.nonIdAuthor | Cagatay, Tolga | - |
dc.contributor.nonIdAuthor | Jiou, Jenny | - |
dc.contributor.nonIdAuthor | Brautigam, Chad A. | - |
dc.contributor.nonIdAuthor | Tomchick, Diana R. | - |
dc.contributor.nonIdAuthor | Sali, Andrej | - |
dc.contributor.nonIdAuthor | D'Arcy, Sheena | - |
dc.contributor.nonIdAuthor | Chook, Yuh Min | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SMALL-ANGLE SCATTERING | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | RESOLUTION STRUCTURE | - |
dc.subject.keywordPlus | DATA-ACQUISITION | - |
dc.subject.keywordPlus | CHAPERONE | - |
dc.subject.keywordPlus | CHROMATIN | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | PROTEIN | - |
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