Probing Conformational Changes of Ubiquitin by Host-Guest Chemistry Using Electrospray Ionization Mass Spectrometry

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dc.contributor.authorLee, Jong Whako
dc.contributor.authorHeo, Sung Wooko
dc.contributor.authorLee, Shin Jung C.ko
dc.contributor.authorKo, Jae Yoonko
dc.contributor.authorKim, Hyungjunko
dc.contributor.authorKim, Hugh I.ko
dc.date.accessioned2019-04-15T15:50:31Z-
dc.date.available2019-04-15T15:50:31Z-
dc.date.created2013-02-26-
dc.date.issued2013-01-
dc.identifier.citationJOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v.24, no.1, pp.21 - 29-
dc.identifier.issn1044-0305-
dc.identifier.urihttp://hdl.handle.net/10203/255292-
dc.description.abstractWe report mechanistic studies of structural changes of ubiquitin (Ub) by host-guest chemistry with cucurbit[6]uril (CB[6]) using electrospray ionization mass spectrometry (ESI-MS) combined with circular dichroism spectroscopy and molecular dynamics (MD) simulation. CB[6] binds selectively to lysine (Lys) residues of proteins. Low energy collision-induced dissociation (CID) of the protein-CB[6] complex reveals CB[6] binding sites. We previously reported (Anal. Chem. 2011, 83, 7916-7923) shifts in major charge states along with Ub-CB[6] complexes in the ESI-MS spectrum with addition of CB[6] to Ub from water. We also reported that CB[6] is present only at Lys(6) or Lys(11) in high charge state (+13) complex. In this study, we provide additional information to explain unique conformational change mechanisms of Ub by host-guest chemistry with CB[6] compared with solvent-driven conformational change of Ub. Additional CID study reveals that CB[6] is bound only to Lys(48) and Lys(63) in low charge state (+7) complex. MD simulation studies reveal that the high charge state complexes are attributed to the CB[6] bound to Lys(11). The complexation prohibits salt bridge formation between Lys(11) and Glu(34) and induces conformational change of Ub. This results in formation of high charge state complexes in the gas phase. Then, by utilizing stronger host-guest chemistry of CB[6] with pentamethylenediamine, refolding of Ub via detaching CB[6] from the protein is performed. Overall, this study gives an insight into the mechanism of denatured Ub ion formation via host-guest interactions with CB[6]. Furthermore, this provides a direction for designing function-controllable supramolecular system comprising proteins and synthetic host molecules.-
dc.languageEnglish-
dc.publisherSPRINGER-
dc.subjectGAS-PHASE-
dc.subjectNONCOVALENT ATTACHMENT-
dc.subjectPROTEIN-STRUCTURE-
dc.subjectION MOBILITY-
dc.subjectSYSTEM-
dc.subjectRECOGNITION-
dc.subjectMECHANISMS-
dc.subjectCOMPLEXES-
dc.subjectDYNAMICS-
dc.subjectDENSITY-
dc.titleProbing Conformational Changes of Ubiquitin by Host-Guest Chemistry Using Electrospray Ionization Mass Spectrometry-
dc.typeArticle-
dc.identifier.wosid000314044300003-
dc.identifier.scopusid2-s2.0-84873042766-
dc.type.rimsART-
dc.citation.volume24-
dc.citation.issue1-
dc.citation.beginningpage21-
dc.citation.endingpage29-
dc.citation.publicationnameJOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY-
dc.identifier.doi10.1007/s13361-012-0496-6-
dc.contributor.localauthorKim, Hyungjun-
dc.contributor.nonIdAuthorLee, Jong Wha-
dc.contributor.nonIdAuthorHeo, Sung Woo-
dc.contributor.nonIdAuthorLee, Shin Jung C.-
dc.contributor.nonIdAuthorKo, Jae Yoon-
dc.contributor.nonIdAuthorKim, Hugh I.-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorUbiquitin-
dc.subject.keywordAuthorConformational change-
dc.subject.keywordAuthorSupramolecular chemistry-
dc.subject.keywordAuthorHost-guest chemistry-
dc.subject.keywordAuthorCucurbit[6] uril-
dc.subject.keywordAuthorElectrospray ionization-
dc.subject.keywordAuthorCollision-induced dissociation-
dc.subject.keywordPlusGAS-PHASE-
dc.subject.keywordPlusNONCOVALENT ATTACHMENT-
dc.subject.keywordPlusPROTEIN-STRUCTURE-
dc.subject.keywordPlusION MOBILITY-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusRECOGNITION-
dc.subject.keywordPlusMECHANISMS-
dc.subject.keywordPlusCOMPLEXES-
dc.subject.keywordPlusDYNAMICS-
dc.subject.keywordPlusDENSITY-
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