DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jaehoon | ko |
dc.contributor.author | Kim, Jung-Ae | ko |
dc.contributor.author | McGinty, Robert K. | ko |
dc.contributor.author | Nguyen, Uyen T. T. | ko |
dc.contributor.author | Muir, Tom W. | ko |
dc.contributor.author | Allis, C. David | ko |
dc.contributor.author | Roeder, Robert G. | ko |
dc.date.accessioned | 2019-04-15T15:32:49Z | - |
dc.date.available | 2019-04-15T15:32:49Z | - |
dc.date.created | 2013-04-18 | - |
dc.date.issued | 2013-03 | - |
dc.identifier.citation | MOLECULAR CELL, v.49, no.6, pp.1121 - 1133 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/255073 | - |
dc.description.abstract | Past studies have documented a crosstalk between H2B ubiquitylation (H2Bub) and H3K4 methylation, but little (if any) direct evidence exists explaining the mechanism underlying H2Bub-dependent H3K4 methylation on chromatin templates. Here, we took advantage of an in vitro histone methyltransferase assay employing a reconstituted yeast Set1 complex (ySet1C) and a recombinant chromatin template containing fully ubiquitylated H2B to gain valuable insights. Combined with genetic analyses, we demonstrate that the n-SET domain within Set1, but not Swd2, is essential for H2Bub-dependent H3K4 methylation. Spp1, a homolog of human CFP1, is conditionally involved in this crosstalk. Our findings extend to the human Set1 complex, underscoring the conserved nature of this disease-relevant crosstalk pathway. As not all members of the H3K4 methyltransferase family contain n-SET domains, our studies draw attention to the n-SET domain as a predictor of an H2B ubiquitylation-sensing mechanism that leads to downstream H3K4 methylation. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.subject | MIXED LINEAGE LEUKEMIA | - |
dc.subject | HISTONE METHYLTRANSFERASE COMPLEX | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | LYSINE-4 METHYLATION | - |
dc.subject | TRANSCRIPTION TERMINATION | - |
dc.subject | ACTIVE GENES | - |
dc.subject | UBIQUITINATION | - |
dc.subject | PROTEIN | - |
dc.subject | TRIMETHYLATION | - |
dc.subject | COMPASS | - |
dc.title | The n-SET Domain of Set1 Regulates H2B Ubiquitylation-Dependent H3K4 Methylation | - |
dc.type | Article | - |
dc.identifier.wosid | 000317024500012 | - |
dc.identifier.scopusid | 2-s2.0-84875771630 | - |
dc.type.rims | ART | - |
dc.citation.volume | 49 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1121 | - |
dc.citation.endingpage | 1133 | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2013.01.034 | - |
dc.contributor.localauthor | Kim, Jaehoon | - |
dc.contributor.nonIdAuthor | Kim, Jung-Ae | - |
dc.contributor.nonIdAuthor | McGinty, Robert K. | - |
dc.contributor.nonIdAuthor | Nguyen, Uyen T. T. | - |
dc.contributor.nonIdAuthor | Muir, Tom W. | - |
dc.contributor.nonIdAuthor | Allis, C. David | - |
dc.contributor.nonIdAuthor | Roeder, Robert G. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MIXED LINEAGE LEUKEMIA | - |
dc.subject.keywordPlus | HISTONE METHYLTRANSFERASE COMPLEX | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | LYSINE-4 METHYLATION | - |
dc.subject.keywordPlus | TRANSCRIPTION TERMINATION | - |
dc.subject.keywordPlus | ACTIVE GENES | - |
dc.subject.keywordPlus | UBIQUITINATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | TRIMETHYLATION | - |
dc.subject.keywordPlus | COMPASS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.