NOVEL SYNTHESIS-REGULATING SRNA AND METHOD FOR PREPARING SAME유전자 발현 조절 기능의 맞춤형 합성 sRNA 제조 방법 및 이를 이용한 tyrosine (그리고 phenol) 및 cadaverine 생성능이 증가된 재조합 미생물 제조 방법

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The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.
Assignee
KAIST
Country
IT (Italy)
Issue Date
2018-11-07
Application Date
2014-07-18
Application Number
13735942.8
Registration Date
2018-11-07
Registration Number
50 2019 000008375
URI
http://hdl.handle.net/10203/254031
Appears in Collection
CBE-Patent(특허)
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