DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Inae | ko |
dc.contributor.author | Jeon, Aeran | ko |
dc.contributor.author | Lim, Suk Gyu | ko |
dc.contributor.author | Hong, Duk Ki | ko |
dc.contributor.author | Kim, Min Soo | ko |
dc.contributor.author | Jo, Jae Hyeong | ko |
dc.contributor.author | Lee, Sang Tak | ko |
dc.contributor.author | Moon, Bongjin | ko |
dc.contributor.author | Oh, Han Bin | ko |
dc.date.accessioned | 2019-04-15T14:15:23Z | - |
dc.date.available | 2019-04-15T14:15:23Z | - |
dc.date.created | 2019-04-03 | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v.30, no.3, pp.538 - 547 | - |
dc.identifier.issn | 1044-0305 | - |
dc.identifier.uri | http://hdl.handle.net/10203/253993 | - |
dc.description.abstract | Free radical-initiated peptide sequencing mass spectrometry (FRIPS MS) was employed to analyze a number of representative singly or doubly protonated phosphopeptides (phosphoserine and phosphotyrosine peptides) in positive ion mode. In contrast to collision-activated dissociation (CAD) results, a loss of a phosphate group occurred to a limited degree for both phosphoserine and phosphotyrosine peptides, and thus, localization of a phosphorylated site was readily achieved. Considering that FRIPS MS supplies a substantial amount of collisional energy to peptides, this result was quite unexpected because a labile phosphate group was conserved. Analysis of the resulting peptide fragments revealed the extensive production of a-, c-, x-, and z-type fragments (with some minor b- and y-type fragments), suggesting that radical-driven peptide fragmentation was the primary mechanism involved in the FRIPS MS of phosphopeptides. Results of this study clearly indicate that FRIPS MS is a promising tool for the characterization of post-translational modifications such as phosphorylation. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | Free Radical-Initiated Peptide Sequencing Mass Spectrometry for Phosphopeptide Post-translational Modification Analysis | - |
dc.type | Article | - |
dc.identifier.wosid | 000461325300016 | - |
dc.identifier.scopusid | 2-s2.0-85062886566 | - |
dc.type.rims | ART | - |
dc.citation.volume | 30 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 538 | - |
dc.citation.endingpage | 547 | - |
dc.citation.publicationname | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | - |
dc.identifier.doi | 10.1007/s13361-018-2100-1 | - |
dc.contributor.localauthor | Jo, Jae Hyeong | - |
dc.contributor.nonIdAuthor | Jang, Inae | - |
dc.contributor.nonIdAuthor | Jeon, Aeran | - |
dc.contributor.nonIdAuthor | Lim, Suk Gyu | - |
dc.contributor.nonIdAuthor | Hong, Duk Ki | - |
dc.contributor.nonIdAuthor | Kim, Min Soo | - |
dc.contributor.nonIdAuthor | Lee, Sang Tak | - |
dc.contributor.nonIdAuthor | Moon, Bongjin | - |
dc.contributor.nonIdAuthor | Oh, Han Bin | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Free radical-initiated peptide sequencing (FRIPS) | - |
dc.subject.keywordAuthor | Radical-driven tandem mass spectrometry | - |
dc.subject.keywordAuthor | Phosphopeptides | - |
dc.subject.keywordAuthor | Phosphorylation | - |
dc.subject.keywordAuthor | Post-translational modifications (PTMs) | - |
dc.subject.keywordPlus | ELECTRON-CAPTURE DISSOCIATION | - |
dc.subject.keywordPlus | INFRARED MULTIPHOTON DISSOCIATION | - |
dc.subject.keywordPlus | DISULFIDE BOND-CLEAVAGE | - |
dc.subject.keywordPlus | DETACHMENT DISSOCIATION | - |
dc.subject.keywordPlus | BACKBONE DISSOCIATIONS | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | FRIPS | - |
dc.subject.keywordPlus | FRAGMENTATION | - |
dc.subject.keywordPlus | CATIONS | - |
dc.subject.keywordPlus | ENERGETICS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.