DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Montell, C | ko |
dc.date.accessioned | 2019-03-19T01:37:22Z | - |
dc.date.available | 2019-03-19T01:37:22Z | - |
dc.date.created | 2019-03-06 | - |
dc.date.issued | 2004-12 | - |
dc.identifier.citation | CURRENT BIOLOGY, v.14, no.23, pp.2076 - 2085 | - |
dc.identifier.issn | 0960-9822 | - |
dc.identifier.uri | http://hdl.handle.net/10203/251731 | - |
dc.description.abstract | Background: Continuous exposure to light, even at relatively low intensities, leads to retinal damage and blindness in wild-type animals. However, the molecular mechanisms underlying constant-light-induced blindness are poorly understood. It has been presumed that the visual impairment resulting from long-term, continuous exposure to ambient light is a secondary consequence of the effects of light on retinal morphology, but this has not been addressed. Results: To characterize the mechanism underlying light-induced blindness, we applied a molecular genetic approach using the fruit fly, Drosophila melanogaster. We found that the temporal loss of the photoresponse was paralleled by a gradual decline in the concentration of rhodopsin. The decline in rhodopsin and the visual response were suppressed by a C-terminal truncation of rhodopsin, by mutations in arrestin, and by elimination of a lysosomal protein, Sunglasses. Conversely, the visual impairment was greatly enhanced by mutation of the rhodopsin phosphatase, rdgC. Surprisingly, the mutations that suppressed light-induced blindness did not reduce the severity of the retinal degeneration resulting from constant light. Moreover, mutations known to suppress retinal degeneration did not ameliorate the light-induced blindness. Conclusions: These data demonstrate that the constant light-induced blindness and retinal degeneration result from defects in distinct molecular pathways. Our results support a model in which visual impairment caused by continuous illumination occurs through an arrestin-dependent pathway that promotes degradation of rhodopsin. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Suppression of constant-light-induced blindness but not retinal degeneration by inhibition of the rhodopsin degradation pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000225810900016 | - |
dc.identifier.scopusid | 2-s2.0-10344229471 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 23 | - |
dc.citation.beginningpage | 2076 | - |
dc.citation.endingpage | 2085 | - |
dc.citation.publicationname | CURRENT BIOLOGY | - |
dc.identifier.doi | 10.1016/j.cub.2004.11.054 | - |
dc.contributor.localauthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Montell, C | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PHOTORECEPTOR CELL | - |
dc.subject.keywordPlus | DROSOPHILA-MELANOGASTER | - |
dc.subject.keywordPlus | VISUAL TRANSDUCTION | - |
dc.subject.keywordPlus | PDZ PROTEIN | - |
dc.subject.keywordPlus | RAT RETINA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | ARRESTIN | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | TRP | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.