Improved production of a bioadhesive precursor protein by fed-batch cultivation of a recombinant Escherichia coli with a pLysS vector

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In batch cultivation, growth of a recombinant Escherichia coli with an inducible T7 expression system and maximum expression of a bioadhesive precursor (BP) protein was similar in the strains with and without the plasmid vector, pLysS. In fed-batch cultivation, however, the strain harboring pLysS grew slower and had a lower level of BP protein expression than that obtained with the strain without pLysS. This suggests that the presence of pLysS in the T7 expression system strongly affects the cell growth and expression of BP protein in high cell density cultivation.
Publisher
Springer Verlag
Issue Date
1998-08
Citation

Biotechnology Letters, Vol.20, No.8, pp.799-803

URI
http://hdl.handle.net/10203/25160
Appears in Collection
CBE-Journal Papers(저널논문)

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