DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Yoon-Seok | ko |
dc.contributor.author | Lee, Jung-Eun | ko |
dc.contributor.author | Nam, Seung-Joo | ko |
dc.contributor.author | Park, Jung-Tak | ko |
dc.contributor.author | Kim, Hyon-Suk | ko |
dc.contributor.author | Choi, Kyu-Hun | ko |
dc.contributor.author | Kim, Beom-Seok | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.date.accessioned | 2019-03-09T05:39:04Z | - |
dc.date.available | 2019-03-09T05:39:04Z | - |
dc.date.created | 2013-07-08 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | PLOS ONE, v.8, no.4 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/251241 | - |
dc.description.abstract | In hepatitis C Virus (HCV) high-risk groups, HCV-specific T cell responses have been detected in seronegative, aviremic persons who have no evidence of HCV infection. Herein, we investigated functional profiles of HCV-specific T-cell responses in seronegative, aviremic patients of a HCV high-risk group. Seventy seven hemodialysis patients with chronic renal disease were analyzed by IFN-gamma ELISpot assays, and eight of 71 (11.3%) seronegative, aviremic patients displayed HCV-specific T-cell responses. Their HCV-specific memory T cells were characterized by assessing cytokine polyfunctionality, known to provide antiviral protection. By intracellular staining of IFN-gamma, TNF-alpha, IL- 2 and MIP-1 beta, we identified two distinct populations in the seronegative, aviremic patients: polyfunctional responders and TNF-alpha-predominant responders. In further analysis, occult HCV infection was excluded as a cause of the HCV-specific T cell response via secondary nested RT-PCR of HCV RNA in peripheral blood mononuclear cell samples. HCV-specific T cells targeted multiple epitopes including non-structural proteins in a single patient, implying that their T cells might have been primed by HCV proteins synthesized within the host. We conclude that HCV-specific memory T cells of seronegative, aviremic patients arise from authentic HCV replication in the host, but not from current occult HCV infection. By functional pattern of HCV-specific T cells, there are two distinct populations in these patients: polyfunctional responders and TNF-alpha-predominant responders. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | BLOOD-DONORS | - |
dc.subject | DETECTABLE VIREMIA | - |
dc.subject | INFECTION | - |
dc.subject | HCV | - |
dc.subject | EXPOSURE | - |
dc.subject | LIVER | - |
dc.subject | SEROCONVERSION | - |
dc.subject | PERSISTENCE | - |
dc.subject | CHIMPANZEES | - |
dc.title | Two Distinct Functional Patterns of Hepatitis C Virus (HCV)-Specific T Cell Responses in Seronegative, Aviremic Patients | - |
dc.type | Article | - |
dc.identifier.wosid | 000319077300042 | - |
dc.identifier.scopusid | 2-s2.0-84876987415 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 4 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0062319 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Lee, Jung-Eun | - |
dc.contributor.nonIdAuthor | Park, Jung-Tak | - |
dc.contributor.nonIdAuthor | Kim, Hyon-Suk | - |
dc.contributor.nonIdAuthor | Choi, Kyu-Hun | - |
dc.contributor.nonIdAuthor | Kim, Beom-Seok | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | BLOOD-DONORS | - |
dc.subject.keywordPlus | DETECTABLE VIREMIA | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | HCV | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | SEROCONVERSION | - |
dc.subject.keywordPlus | PERSISTENCE | - |
dc.subject.keywordPlus | CHIMPANZEES | - |
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