Photophysical properties of Zn-substituted cytochrome c investigated by single-molecule and ensemble-averaged spectroscopy

Abstract

The study of the structural reorganization and photophysical properties of Zn-Cytc using the single-molecule and ensemble-averaged spectroscopy shows that the photoblinking behaviors of single-Zn-Cytc depend on the folded and unfolded structures, whereas the fluorescence dynamics of Zn-Cytc observed in the bulk phase are hardly affected by the conformational change of a protein.