DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Jungkweon | ko |
dc.contributor.author | Yang, Cheolhee | ko |
dc.contributor.author | Kim, Jeongho | ko |
dc.contributor.author | Ihee, Hyotcherl | ko |
dc.date.accessioned | 2019-03-07T12:13:41Z | - |
dc.date.available | 2019-03-07T12:13:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | JOURNAL OF PHYSICAL CHEMISTRY B, v.115, no.12, pp.3127 - 3135 | - |
dc.identifier.issn | 1520-6106 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250900 | - |
dc.description.abstract | We investigate optically triggered protein folding dynamics of cytochrome c (Cytc) using transient grating (TG) and transient absorption (TA) spectroscopies. Despite many studies on protein folding dynamics of Cytc, a well-known model protein, direct spectroscopic evidence for the three-dimensional global folding process has been rarely reported. By measuring the TG signal of CO-bound Cytc (Cytc-CO) in the presence of a denaturant, we clearly detected the change of diffusion coefficient that reflects the size change of Cytc upon photodissociation of the CO ligand from unfolded Cytc-CO. The quantitative analysis of TG signals supports that the optically triggered folding reaction of Cytc in the presence of a denaturant takes place through a detectable intermediate (three-state folding kinetics). This is in contrast with the two-state folding dynamics of Cytc under a denaturant-free environment without any detectable intermediate.(1) From the quantitative global analysis of the TG signals, the rate constants for the U -> I and I -> N transitions in a CAPS buffer solution (pH 7) at room temperature in the presence of a denaturant at various concentrations are determined to be 1065 +/- 17 to 3476 +/- 103 s(-1) and 101 +/- 6 to 589 +/- 21 s(-1), respectively. In addition, the activation energies (E(a)) for the U -> I and I -> N transitions are determined to be 8.7 +/- 1.0 kcal/mol and 7.1 +/- 1.3 kcal/mol, respectively. The folding dynamics of Cytc initiated by the CO photolysis is discussed based in terms of the protein size change. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | RESOLVED CIRCULAR-DICHROISM | - |
dc.subject | X-RAY-SCATTERING | - |
dc.subject | FAST EVENTS | - |
dc.subject | INTERMOLECULAR INTERACTION | - |
dc.subject | DIFFUSION-COEFFICIENT | - |
dc.subject | ELECTRON-TRANSFER | - |
dc.subject | CARBON-MONOXIDE | - |
dc.subject | KINETICS | - |
dc.subject | DENATURATION | - |
dc.subject | MOLECULES | - |
dc.title | Protein Folding Dynamics of Cytochrome c Seen by Transient Grating and Transient Absorption Spectroscopies | - |
dc.type | Article | - |
dc.identifier.wosid | 000288644700050 | - |
dc.identifier.scopusid | 2-s2.0-79953037296 | - |
dc.type.rims | ART | - |
dc.citation.volume | 115 | - |
dc.citation.issue | 12 | - |
dc.citation.beginningpage | 3127 | - |
dc.citation.endingpage | 3135 | - |
dc.citation.publicationname | JOURNAL OF PHYSICAL CHEMISTRY B | - |
dc.identifier.doi | 10.1021/jp106588d | - |
dc.contributor.localauthor | Ihee, Hyotcherl | - |
dc.contributor.nonIdAuthor | Choi, Jungkweon | - |
dc.contributor.nonIdAuthor | Kim, Jeongho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RESOLVED CIRCULAR-DICHROISM | - |
dc.subject.keywordPlus | X-RAY-SCATTERING | - |
dc.subject.keywordPlus | FAST EVENTS | - |
dc.subject.keywordPlus | INTERMOLECULAR INTERACTION | - |
dc.subject.keywordPlus | DIFFUSION-COEFFICIENT | - |
dc.subject.keywordPlus | ELECTRON-TRANSFER | - |
dc.subject.keywordPlus | CARBON-MONOXIDE | - |
dc.subject.keywordPlus | KINETICS | - |
dc.subject.keywordPlus | DENATURATION | - |
dc.subject.keywordPlus | MOLECULES | - |
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