DC Field | Value | Language |
---|---|---|
dc.contributor.author | Joo, Sunghoon | ko |
dc.contributor.author | Nam, Yoonkey | ko |
dc.date.accessioned | 2019-02-20T05:11:49Z | - |
dc.date.available | 2019-02-20T05:11:49Z | - |
dc.date.created | 2019-02-11 | - |
dc.date.created | 2019-02-11 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, v.66, no.2, pp.403 - 410 | - |
dc.identifier.issn | 0018-9294 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250351 | - |
dc.description.abstract | Objective: The use of microelectrode array (MEA) recordings is a very effective neurophysiological method because it is able to continuously and noninvasively obtain the spatiotemporal information of electrical activity from many neurons constituting a neural network. Very recently, studies have been published that used MEAs for the measurement of a low-frequency component of electrical activity as an indicator of diverse activity of cultured neurons. The occurrence of low-frequency activities has electrophysiological information that does not include the information from fast spikes. However, there is no in vitro experimental model suitable for measuring the low-frequency activities (slow-waves) for further study. Methods: Neural clusters consisting of dozens of neurons were placed directly onto each electrode of an MEA from which fast spikes and slow-waves were measured. Results: We obtained sufficient data on the early development patterns of the slow-waves and the spikes measured from many independent neural clusters confirming that the slow-waves occurred first before the emergence of the spikes in the neural clusters. We also showed that changes in the occurrence frequency of the slow-waves for synaptic blockers were measured from a large number of independent cultures. Conclusion: Microsized neural cluster arrays, which can be combined with conventional MEAs, are suitable for multiple simultaneous recordings of slow-waves. Significance: Our technology provides a simple but useful method to study the generation of a low-frequency component of the electrical activity in cultured neural networks that are not yet well known as well as to expand the use of conventional MEAs. | - |
dc.language | English | - |
dc.publisher | IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC | - |
dc.title | Slow-Wave Recordings From Micro-Sized Neural Clusters Using Multiwell Type Microelectrode Arrays | - |
dc.type | Article | - |
dc.identifier.wosid | 000456915400012 | - |
dc.identifier.scopusid | 2-s2.0-85047968238 | - |
dc.type.rims | ART | - |
dc.citation.volume | 66 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 403 | - |
dc.citation.endingpage | 410 | - |
dc.citation.publicationname | IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING | - |
dc.identifier.doi | 10.1109/TBME.2018.2843793 | - |
dc.contributor.localauthor | Nam, Yoonkey | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Cell patterning | - |
dc.subject.keywordAuthor | electrophysiology | - |
dc.subject.keywordAuthor | neural recording | - |
dc.subject.keywordAuthor | microelectrode arrays | - |
dc.subject.keywordAuthor | cell culture | - |
dc.subject.keywordPlus | NETWORKS | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | SPIKES | - |
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