DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Nam Hee | ko |
dc.contributor.author | Nahm, Seung-Hoon | ko |
dc.contributor.author | Choi, Insung S. | ko |
dc.date.accessioned | 2019-02-20T04:52:17Z | - |
dc.date.available | 2019-02-20T04:52:17Z | - |
dc.date.created | 2019-01-28 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | SENSORS, v.18, no.12 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250222 | - |
dc.description.abstract | The possibility of exposure to botulinum neurotoxin (BoNT), a powerful and potential bioterrorism agent, is considered to be ever increasing. The current gold-standard assay, live-mouse lethality, exhibits high sensitivity but has limitations including long assay times, whereas other assays evince rapidity but lack factors such as real-time monitoring or portability. In this study, we aimed to devise a novel detection system that could detect BoNT at below-nanomolar concentrations in the form of a stretchable biosensor. We used a field-effect transistor with a p-type channel and electrodes, along with a channel comprising aligned carbon nanotube layers to detect the type E light chain of BoNT (BoNT/E-Lc). The detection of BoNT/E-Lc entailed observing the cleavage of a unique peptide and the specific bonding between BoNT/E-Lc and antibody BoNT/E-Lc (Anti-BoNT/E-Lc). The unique peptide was cleaved by 60 pM BoNT/E-Lc; notably, 52 fM BoNT/E-Lc was detected within 1 min in the device with the antibody in the bent state. These results demonstrated that an all-carbon nanotube-based device (all-CNT-based device) could be produced without a complicated fabrication process and could be used as a biosensor with high sensitivity, suggesting its potential development as a wearable BoNT biosensor. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.title | Real-Time Monitoring of a Botulinum Neurotoxin Using All-Carbon Nanotube-Based Field-Effect Transistor Devices | - |
dc.type | Article | - |
dc.identifier.wosid | 000454817100154 | - |
dc.identifier.scopusid | 2-s2.0-85058081215 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 12 | - |
dc.citation.publicationname | SENSORS | - |
dc.identifier.doi | 10.3390/s18124235 | - |
dc.contributor.localauthor | Choi, Insung S. | - |
dc.contributor.nonIdAuthor | Nahm, Seung-Hoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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