DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jin Woo | - |
dc.contributor.author | Won, Jungyeon | - |
dc.contributor.author | Sohn, Seonghyang | - |
dc.contributor.author | Joe, Cheol O. | - |
dc.date.accessioned | 2011-08-30T01:09:21Z | - |
dc.date.available | 2011-08-30T01:09:21Z | - |
dc.date.issued | 2000-03 | - |
dc.identifier.citation | Journal of Cell Science, Vol.113, pp.955-961 | en |
dc.identifier.uri | http://hdl.handle.net/10203/25015 | - |
dc.description.abstract | The role of the N-terminal cleavage product of poly(ADP-ribose) polymerase (PARP) on UV mediated apoptosis was investigated in cultured HeLa cells. Ultrastructural analysis of cells expressing caspase-resistant PARP (PARP(D214A)) revealed the typical features of necrosis following UV treatment. However, cells co-expressing PARP(D214A) with the N-terminal fragment of PARP containing the DNA-binding domain underwent apoptosis instead of necrosis. In this study, we have demonstrated that the DNA-binding activity of the N-terminal fragment of PARP is important for the execution of apoptosis. Point mutations were introduced in the DNA-binding sites of the N-terminal fragment. Cells co-expressing PARP(D214A) with the mutated N-terminal fragments neither stimulated apoptosis nor prevented necrosis in response to UV irradiation. The present study proposes that the DNA-binding activity of the N-terminal fragment of PARP in UV treated cells prevents cellular ATP depletion, a mechanism by which necrotic cell death is triggered. | en |
dc.description.sponsorship | This work was supported in part by a Grant from the Korea Science and Engineering Foundation, a Grant from Ministry of Science and Technology, and a Grant from the Ministry of Education (Genetic Engineering Program), South Korea. | en |
dc.language.iso | en_US | en |
dc.publisher | Company of Biologists | en |
dc.subject | Cell death | en |
dc.subject | Apopotis | en |
dc.subject | Necrosis | en |
dc.subject | Poly(ADP-ribose) polymerase | en |
dc.subject | ATP depletion | en |
dc.title | DNA-Binding activity of the N-terminal cleavage product of poly(ADP-ribose) polymerase is required for UV mediated apoptosis. | en |
dc.type | Article | en |
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