Identification and Characterization of eEF1BδL as a Novel Ubiquitylation Target of RNF20/40

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 230
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorIn, Sunako
dc.contributor.authorKim, Yong-Inko
dc.contributor.authorLee, J. Eugeneko
dc.contributor.authorKim, Jaehoonko
dc.date.accessioned2019-01-23T05:26:22Z-
dc.date.available2019-01-23T05:26:22Z-
dc.date.created2018-12-27-
dc.date.issued2018-09-06-
dc.identifier.citation2018 EMBO EMBL Symposia: Principles of Chromosome Structure and Function-
dc.identifier.urihttp://hdl.handle.net/10203/249431-
dc.description.abstractIn mammalian cells, the RING-finger type E3 ubiquitin ligase RNF20/40 catalyzes mono-ubiquitylation at lysine 120 of histone H2B that is implicated in transcriptional regulation. Previous studies have shown that depletion of RNF20/40 causes defects in various cellular events, suggesting that RNF20/40 may have additional ubiquitylation target proteins other than histone H2B. In order to find novel non-histone target proteins of RNF20/40, we performed co-immunoprecipitation using FLAG-RNF20 cell line. Subsequent mass spectrometry analysis identified eEF1BδL, a DNA binding transcription factor that is previously known to regulate expression of heat shock responsive genes, as an RNF20/40-interacting protein. In an in vitro ubiqtuiatylaion assay using purified factors, we found that eEF1BδL can be ubiquitylated by RNF20/40 and RAD6-dependent manner. In addition, we further found that RNF20/40-dependent ubiquitylation of eEF1BδL enhances expression of heat shock responsive genes with underlying mechanism that involves a cooperative interaction of eEF1BδL and RNF20/40 with HSF1. Collectively, our study demonstrates a novel transcriptional regulation pathway of heat shock responsive genes via ubiquitylation of eEF1BδL by RNF20/40.-
dc.languageEnglish-
dc.publisherEuropean Molecular Biology Organization-
dc.titleIdentification and Characterization of eEF1BδL as a Novel Ubiquitylation Target of RNF20/40-
dc.typeConference-
dc.type.rimsCONF-
dc.citation.publicationname2018 EMBO EMBL Symposia: Principles of Chromosome Structure and Function-
dc.identifier.conferencecountryGE-
dc.identifier.conferencelocationHeidelberg-
dc.contributor.localauthorKim, Jaehoon-
dc.contributor.nonIdAuthorKim, Yong-In-
dc.contributor.nonIdAuthorLee, J. Eugene-
Appears in Collection
BS-Conference Papers(학술회의논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0