DC Field | Value | Language |
---|---|---|
dc.contributor.author | In, Suna | ko |
dc.contributor.author | Kim, Yong-In | ko |
dc.contributor.author | Lee, J. Eugene | ko |
dc.contributor.author | Kim, Jaehoon | ko |
dc.date.accessioned | 2019-01-23T05:26:22Z | - |
dc.date.available | 2019-01-23T05:26:22Z | - |
dc.date.created | 2018-12-27 | - |
dc.date.issued | 2018-09-06 | - |
dc.identifier.citation | 2018 EMBO EMBL Symposia: Principles of Chromosome Structure and Function | - |
dc.identifier.uri | http://hdl.handle.net/10203/249431 | - |
dc.description.abstract | In mammalian cells, the RING-finger type E3 ubiquitin ligase RNF20/40 catalyzes mono-ubiquitylation at lysine 120 of histone H2B that is implicated in transcriptional regulation. Previous studies have shown that depletion of RNF20/40 causes defects in various cellular events, suggesting that RNF20/40 may have additional ubiquitylation target proteins other than histone H2B. In order to find novel non-histone target proteins of RNF20/40, we performed co-immunoprecipitation using FLAG-RNF20 cell line. Subsequent mass spectrometry analysis identified eEF1BδL, a DNA binding transcription factor that is previously known to regulate expression of heat shock responsive genes, as an RNF20/40-interacting protein. In an in vitro ubiqtuiatylaion assay using purified factors, we found that eEF1BδL can be ubiquitylated by RNF20/40 and RAD6-dependent manner. In addition, we further found that RNF20/40-dependent ubiquitylation of eEF1BδL enhances expression of heat shock responsive genes with underlying mechanism that involves a cooperative interaction of eEF1BδL and RNF20/40 with HSF1. Collectively, our study demonstrates a novel transcriptional regulation pathway of heat shock responsive genes via ubiquitylation of eEF1BδL by RNF20/40. | - |
dc.language | English | - |
dc.publisher | European Molecular Biology Organization | - |
dc.title | Identification and Characterization of eEF1BδL as a Novel Ubiquitylation Target of RNF20/40 | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.citation.publicationname | 2018 EMBO EMBL Symposia: Principles of Chromosome Structure and Function | - |
dc.identifier.conferencecountry | GE | - |
dc.identifier.conferencelocation | Heidelberg | - |
dc.contributor.localauthor | Kim, Jaehoon | - |
dc.contributor.nonIdAuthor | Kim, Yong-In | - |
dc.contributor.nonIdAuthor | Lee, J. Eugene | - |
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