Single-Molecule Rotation for EGFR Conformational Dynamics in Live Cells

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Monitoring the dynamics of proteins in live cells on appropriate spatiotemporal scales may provide key information regarding long-standing questions in molecular and cellular regulatory mechanisms. However, tools capable of imaging the conformational changes over time have been elusive. Here, we present a single-molecule stroboscopic imaging probes by developing gyroscopic plasmonic nanoparticles, allowing for replication of protein protein interactions and the conformational dynamics based on rotational and lateral velocities. This study fundamentally monitors the rotational motion of a membrane protein, epidermal growth factor receptor (EGFR), to decipher undiscovered structural dynamics in live cells without any molecular perturbations. This method offers a strategy to visualize assemblies and conformational changes, and provides unique insights into the mechanism underlying the molecular dynamics for receptors.
Publisher
AMER CHEMICAL SOC
Issue Date
2018-11
Language
English
Article Type
Article
Citation

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.140, no.45, pp.15161 - 15165

ISSN
0002-7863
DOI
10.1021/jacs.8b09037
URI
http://hdl.handle.net/10203/248277
Appears in Collection
CH-Journal Papers(저널논문)
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