DC Field | Value | Language |
---|---|---|
dc.contributor.author | Martinez-Avila, Olga | ko |
dc.contributor.author | Wu, Shenping | ko |
dc.contributor.author | Kim, Seung Joong | ko |
dc.contributor.author | Cheng, Yifan | ko |
dc.contributor.author | Khan, Feroz | ko |
dc.contributor.author | Samudrala, Ram | ko |
dc.contributor.author | Sali, Andrej | ko |
dc.contributor.author | Horst, Jeremy A. | ko |
dc.contributor.author | Habelitz, Stefan | ko |
dc.date.accessioned | 2018-10-19T00:43:14Z | - |
dc.date.available | 2018-10-19T00:43:14Z | - |
dc.date.created | 2018-10-01 | - |
dc.date.created | 2018-10-01 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.citation | BIOMACROMOLECULES, v.13, no.11, pp.3494 - 3502 | - |
dc.identifier.issn | 1525-7797 | - |
dc.identifier.uri | http://hdl.handle.net/10203/246076 | - |
dc.description.abstract | Enamel matrix self-assembly has long been,. suggested as the driving force behind aligned nanofibrous hydroxyaptite. formation. We tested if amelogenin, the main enamel matrix protein, can self assemble into ribbon-like structures in physiologic solutions. Ribbons 17 nm wide were,observed to grow several micrometers in length, requiring calcium, phosphate, and pH 4.07-6.0. The pH range suggests that the formation. of ion bridges through protonated histidine residues is essential to self assembly, supported by a statistical analysis of 212 phosphate-binding proteins predicting. 12 phosphate binding histidines. Thermophoretic analysis verified the importance of calcium and Phosphate in self assembly X-ray scattering characterized amelogenin dimers with dimensions fitting the cross-section of the amelogenin ribbon leading to the hypothesis that antiparallel dimers are the building blocks Of the ribbons Over 5-7 days, ribbons self-organized-into bundles composed Of aligned ribbons mimicking the structure of enamel crystallites in enamel rods. These observations confirm reports of filamentous Organic components in developing enamel and provide a new Model for matrix-templated enamel mineralization. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | X-RAY-SCATTERING | - |
dc.subject | ENAMEL MATRIX | - |
dc.subject | IN-VITRO | - |
dc.subject | MICROSCALE THERMOPHORESIS | - |
dc.subject | SECONDARY STRUCTURE | - |
dc.subject | PORCINE AMELOGENIN | - |
dc.subject | SECRETORY STAGE | - |
dc.subject | CRYSTAL-GROWTH | - |
dc.subject | PROTEIN | - |
dc.subject | BINDING | - |
dc.title | Self-Assembly of Filamentous Amelogenin Requires Calcium and Phosphate: From Dimers via Nanoribbons to Fibrils | - |
dc.type | Article | - |
dc.identifier.wosid | 000310931900009 | - |
dc.identifier.scopusid | 2-s2.0-84869057718 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 3494 | - |
dc.citation.endingpage | 3502 | - |
dc.citation.publicationname | BIOMACROMOLECULES | - |
dc.identifier.doi | 10.1021/bm300942c | - |
dc.contributor.localauthor | Kim, Seung Joong | - |
dc.contributor.nonIdAuthor | Martinez-Avila, Olga | - |
dc.contributor.nonIdAuthor | Wu, Shenping | - |
dc.contributor.nonIdAuthor | Cheng, Yifan | - |
dc.contributor.nonIdAuthor | Khan, Feroz | - |
dc.contributor.nonIdAuthor | Samudrala, Ram | - |
dc.contributor.nonIdAuthor | Sali, Andrej | - |
dc.contributor.nonIdAuthor | Horst, Jeremy A. | - |
dc.contributor.nonIdAuthor | Habelitz, Stefan | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | X-RAY-SCATTERING | - |
dc.subject.keywordPlus | ENAMEL MATRIX | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | MICROSCALE THERMOPHORESIS | - |
dc.subject.keywordPlus | SECONDARY STRUCTURE | - |
dc.subject.keywordPlus | PORCINE AMELOGENIN | - |
dc.subject.keywordPlus | SECRETORY STAGE | - |
dc.subject.keywordPlus | CRYSTAL-GROWTH | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BINDING | - |
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