DC Field | Value | Language |
---|---|---|
dc.contributor.author | Algret, Romain | ko |
dc.contributor.author | Fernandez-Martinez, Javier | ko |
dc.contributor.author | Shi, Yi | ko |
dc.contributor.author | Kim, Seung Joong | ko |
dc.contributor.author | Pellarin, Riccardo | ko |
dc.contributor.author | Cimermancic, Peter | ko |
dc.contributor.author | Cochet, Emilie | ko |
dc.contributor.author | Sali, Andrej | ko |
dc.contributor.author | Chait, Brian T. | ko |
dc.contributor.author | Rout, Michael P. | ko |
dc.contributor.author | Dokudovskaya, Svetlana | ko |
dc.date.accessioned | 2018-10-19T00:43:02Z | - |
dc.date.available | 2018-10-19T00:43:02Z | - |
dc.date.created | 2018-10-01 | - |
dc.date.created | 2018-10-01 | - |
dc.date.issued | 2014-11 | - |
dc.identifier.citation | MOLECULAR & CELLULAR PROTEOMICS, v.13, no.11, pp.2855 - 2870 | - |
dc.identifier.issn | 1535-9476 | - |
dc.identifier.uri | http://hdl.handle.net/10203/246068 | - |
dc.description.abstract | The TORC1 signaling pathway plays a major role in the control of cell growth and response to stress. Here we demonstrate that the SEA complex physically interacts with TORC1 and is an important regulator of its activity. During nitrogen starvation, deletions of SEA complex components lead to Tor1 kinase delocalization, defects in autophagy, and vacuolar fragmentation. TORC1 inactivation, via nitrogen deprivation or rapamycin treatment, changes cellular levels of SEA complex members. We used affinity purification and chemical cross-linking to generate the data for an integrative structure modeling approach, which produced a well-defined molecular architecture of the SEA complex and showed that the SEA complex comprises two regions that are structurally and functionally distinct. The SEA complex emerges as a platform that can coordinate both structural and enzymatic activities necessary for the effective functioning of the TORC1 pathway. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | NUCLEAR-PORE COMPLEX | - |
dc.subject | TRANSFER-RNA SYNTHETASE | - |
dc.subject | TUMOR-SUPPRESSOR NPRL2 | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | CROSS-LINKING | - |
dc.subject | RAG GTPASES | - |
dc.subject | MACROMOLECULAR ASSEMBLIES | - |
dc.subject | STRUCTURE PREDICTION | - |
dc.subject | NITROGEN STARVATION | - |
dc.subject | PROTEIN-STRUCTURE | - |
dc.title | Molecular Architecture and Function of the SEA Complex, a Modulator of the TORC1 Pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000344930500006 | - |
dc.identifier.scopusid | 2-s2.0-84910612231 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 2855 | - |
dc.citation.endingpage | 2870 | - |
dc.citation.publicationname | MOLECULAR & CELLULAR PROTEOMICS | - |
dc.identifier.doi | 10.1074/mcp.M114.039388 | - |
dc.contributor.localauthor | Kim, Seung Joong | - |
dc.contributor.nonIdAuthor | Algret, Romain | - |
dc.contributor.nonIdAuthor | Fernandez-Martinez, Javier | - |
dc.contributor.nonIdAuthor | Shi, Yi | - |
dc.contributor.nonIdAuthor | Pellarin, Riccardo | - |
dc.contributor.nonIdAuthor | Cimermancic, Peter | - |
dc.contributor.nonIdAuthor | Cochet, Emilie | - |
dc.contributor.nonIdAuthor | Sali, Andrej | - |
dc.contributor.nonIdAuthor | Chait, Brian T. | - |
dc.contributor.nonIdAuthor | Rout, Michael P. | - |
dc.contributor.nonIdAuthor | Dokudovskaya, Svetlana | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NUCLEAR-PORE COMPLEX | - |
dc.subject.keywordPlus | TRANSFER-RNA SYNTHETASE | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR NPRL2 | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | CROSS-LINKING | - |
dc.subject.keywordPlus | RAG GTPASES | - |
dc.subject.keywordPlus | MACROMOLECULAR ASSEMBLIES | - |
dc.subject.keywordPlus | STRUCTURE PREDICTION | - |
dc.subject.keywordPlus | NITROGEN STARVATION | - |
dc.subject.keywordPlus | PROTEIN-STRUCTURE | - |
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