Iterative expansion microscopy

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dc.contributor.authorChang, Jae-Byumko
dc.contributor.authorChen, Feiko
dc.contributor.authorYoon, Young-Gyuko
dc.contributor.authorJung, Erica E.ko
dc.contributor.authorBabcock, Hazenko
dc.contributor.authorKang, Jeong Seukko
dc.contributor.authorAsano, Shohko
dc.contributor.authorSuk, Ho-Junko
dc.contributor.authorPak, Nikitako
dc.contributor.authorTillberg, Paul W.ko
dc.contributor.authorWassie, Asmamaw T.ko
dc.contributor.authorCai, Dawenko
dc.contributor.authorBoyden, Edward S.ko
dc.date.accessioned2018-09-18T06:24:48Z-
dc.date.available2018-09-18T06:24:48Z-
dc.date.created2018-09-04-
dc.date.created2018-09-04-
dc.date.created2018-09-04-
dc.date.created2018-09-04-
dc.date.issued2017-06-
dc.identifier.citationNATURE METHODS, v.14, no.6, pp.593 - +-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/10203/245588-
dc.description.abstractWe recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by-4.5x in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded-20x. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens similar to 4.5 x 4.5, or similar to 20x, and enables similar to 25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleIterative expansion microscopy-
dc.typeArticle-
dc.identifier.wosid000402291800022-
dc.identifier.scopusid2-s2.0-85017506239-
dc.type.rimsART-
dc.citation.volume14-
dc.citation.issue6-
dc.citation.beginningpage593-
dc.citation.endingpage+-
dc.citation.publicationnameNATURE METHODS-
dc.identifier.doi10.1038/NMETH.4261-
dc.contributor.localauthorChang, Jae-Byum-
dc.contributor.localauthorYoon, Young-Gyu-
dc.contributor.nonIdAuthorChen, Fei-
dc.contributor.nonIdAuthorJung, Erica E.-
dc.contributor.nonIdAuthorBabcock, Hazen-
dc.contributor.nonIdAuthorKang, Jeong Seuk-
dc.contributor.nonIdAuthorAsano, Shoh-
dc.contributor.nonIdAuthorSuk, Ho-Jun-
dc.contributor.nonIdAuthorPak, Nikita-
dc.contributor.nonIdAuthorTillberg, Paul W.-
dc.contributor.nonIdAuthorWassie, Asmamaw T.-
dc.contributor.nonIdAuthorCai, Dawen-
dc.contributor.nonIdAuthorBoyden, Edward S.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
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