Iterative expansion microscopy

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We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by-4.5x in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded-20x. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens similar to 4.5 x 4.5, or similar to 20x, and enables similar to 25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-06
Language
English
Article Type
Article
Citation

NATURE METHODS, v.14, no.6, pp.593 - +

ISSN
1548-7091
DOI
10.1038/NMETH.4261
URI
http://hdl.handle.net/10203/245588
Appears in Collection
MS-Journal Papers(저널논문)EE-Journal Papers(저널논문)
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