Rapid naked-eye detection of Gram-positive bacteria by vancomycin-based nano-aggregation

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Development of a rapid, point-of-care assay for diagnosing bacterial infections is crucial for subsequent treatment of the patient and preventing the overuse of antibiotics. Herein, we describe a rapid, one-step colorimetric assay based on the formation of nano-aggregates using nanobeads targeting Gram-positive bacteria. Vancomycin was immobilized onto blue-colored polymeric nanobeads to induce specific and multivalent binding with the Gram-positive bacterial cell wall and subsequent agglomeration. Without any pre-processing steps, the addition of various types of Gram-positive pathogens to the nanobeads resulted in the formation of blue precipitates, which could be observed with the naked eye in approximate to 30 min. We also utilized a porous filter system for the assay, which allowed discrimination of Gram-positive targets with higher selectivity, and demonstrated feasibility as a simple diagnostic assay with minimal technical components. We anticipate that the nanobead aggregation assay can be potentially applied as a rapid and simple sensing platform, which can be easily miniaturized and enable point-of-care diagnosis of Gram-positive infections.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2018-07
Language
English
Article Type
Article
Keywords

PATHOGENIC BACTERIA; INFECTIOUS-DISEASES; GOLD NANOPARTICLES; ANTIBIOTIC-RESISTANCE; COLORIMETRIC ASSAY; ONE-STEP; DIAGNOSTICS; ACID; PCR; AMPLIFICATION

Citation

RSC ADVANCES , v.8, no.44, pp.25094 - 25103

ISSN
2046-2069
DOI
10.1039/c8ra03540g
URI
http://hdl.handle.net/10203/244881
Appears in Collection
NT-Journal Papers(저널논문)
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