DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hong Won | ko |
dc.contributor.author | Choi, Byung San | ko |
dc.contributor.author | Kang, Han Na | ko |
dc.contributor.author | Kim, Hyun Woo | ko |
dc.contributor.author | Min, Ahrum | ko |
dc.contributor.author | Cha, Minkwon | ko |
dc.contributor.author | Ryu, Ji Young | ko |
dc.contributor.author | Park, Sangwoo | ko |
dc.contributor.author | Sohn, Jinyoung | ko |
dc.contributor.author | Shin, Kihyuk | ko |
dc.contributor.author | Yun, Mi Ran | ko |
dc.contributor.author | Han, Joo Yeun | ko |
dc.contributor.author | Shon, Min Ju | ko |
dc.contributor.author | Jeong, Cherlhyun | ko |
dc.contributor.author | Chung, Junho | ko |
dc.contributor.author | Lee, Seung-Hyo | ko |
dc.contributor.author | Im, Seock-Ah | ko |
dc.contributor.author | Cho, Byoung Chul | ko |
dc.contributor.author | Yoon, Tae-Young | ko |
dc.date.accessioned | 2018-07-24T02:41:21Z | - |
dc.date.available | 2018-07-24T02:41:21Z | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.created | 2018-07-04 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.citation | NATURE BIOMEDICAL ENGINEERING, v.2, no.4, pp.239 - 253 | - |
dc.identifier.issn | 2157-846X | - |
dc.identifier.uri | http://hdl.handle.net/10203/244335 | - |
dc.description.abstract | The accumulation of genetic and epigenetic alterations in cancer cells rewires cellular signalling pathways through changes in the patterns of protein-protein interactions (PPIs). Understanding these patterns may facilitate the design of tailored cancer therapies. Here, we show that single-molecule pull-down and co-immunoprecipitation techniques can be used to characterize signalling complexes of the human epidermal growth-factor receptor (HER) family in specific cancers. By analysing cancer-specific signalling phenotypes, including post-translational modifications and PPIs with downstream interactions, we found that activating mutations of the epidermal growth-factor receptor (EGFR) gene led to the formation of large protein complexes surrounding mutant EGFR proteins and to a reduction in the dependency of mutant EGFR signalling on phosphotyrosine residues, and that the strength of HER-family PPIs is correlated with the strength of the dependence of breast and lung adenocarcinoma cells on HER-family signalling pathways. Furthermore, using co-immunoprecipitation profiling to screen for EGFR-dependent cancers, we identified non-small-cell lung cancers that respond to an EGFR-targeted inhibitor. Our approach might help predict responses to targeted cancer therapies, particularly for cancers that lack actionable genomic mutations. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors | - |
dc.type | Article | - |
dc.identifier.wosid | 000435466700010 | - |
dc.identifier.scopusid | 2-s2.0-85044743214 | - |
dc.type.rims | ART | - |
dc.citation.volume | 2 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 239 | - |
dc.citation.endingpage | 253 | - |
dc.citation.publicationname | NATURE BIOMEDICAL ENGINEERING | - |
dc.identifier.doi | 10.1038/s41551-018-0212-3 | - |
dc.contributor.localauthor | Lee, Seung-Hyo | - |
dc.contributor.localauthor | Yoon, Tae-Young | - |
dc.contributor.nonIdAuthor | Kang, Han Na | - |
dc.contributor.nonIdAuthor | Min, Ahrum | - |
dc.contributor.nonIdAuthor | Park, Sangwoo | - |
dc.contributor.nonIdAuthor | Sohn, Jinyoung | - |
dc.contributor.nonIdAuthor | Yun, Mi Ran | - |
dc.contributor.nonIdAuthor | Han, Joo Yeun | - |
dc.contributor.nonIdAuthor | Shon, Min Ju | - |
dc.contributor.nonIdAuthor | Jeong, Cherlhyun | - |
dc.contributor.nonIdAuthor | Chung, Junho | - |
dc.contributor.nonIdAuthor | Im, Seock-Ah | - |
dc.contributor.nonIdAuthor | Cho, Byoung Chul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL LUNG-CANCER | - |
dc.subject.keywordPlus | KINASE DOMAIN | - |
dc.subject.keywordPlus | INTERACTION NETWORK | - |
dc.subject.keywordPlus | TYROSINE KINASES | - |
dc.subject.keywordPlus | LIGATION ASSAYS | - |
dc.subject.keywordPlus | BINDING-SITES | - |
dc.subject.keywordPlus | SCALE MAP | - |
dc.subject.keywordPlus | PULL-DOWN | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | GEFITINIB | - |
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