Regulation of chromatin architecture by CHD4 in embryonic stem cells

Abstract

The positioning of the nucleosome by ATP‐dependent chromatin remodelers provides the fundamental chromatin environment for the regulation of diverse cellular processes acting on the underlying DNA. Recently, genome‐wide nucleosome mapping has revealed more detailed information on the chromatin‐remodeling factors. Using genome-wide approaches, we analyzed total RNA transcriptomes, mRNA transcriptomes, histone modifications ChIP-seq, nucleosome positioning, ATAC-seq and Hi-C profiles by knockdown of chromatin remodeler in mouse embryonic stem cells. In this talk, I will describe how chromatin remodeler regulates nucleosome occupancy and how these activities are linked to 3D architecture of embryonic stem cell to maintain pluripotency. Finally, I will discuss the recent progress about the potential relevance of 3D architecture for regulating chromatin stability in mouse embryonic stem cells.