DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Mina | ko |
dc.contributor.author | Shin, Soochul | ko |
dc.contributor.author | Uhm, Heesoo | ko |
dc.contributor.author | Hong, Heesun | ko |
dc.contributor.author | Kirk, Jaewon | ko |
dc.contributor.author | Hyun, Kwang Beom | ko |
dc.contributor.author | Kulikowicz, Tomasz | ko |
dc.contributor.author | Kim, Jaehoon | ko |
dc.contributor.author | Ahn, Byungchan | ko |
dc.contributor.author | Bohr, Vilhelm A. | ko |
dc.contributor.author | Hohng, Sungchul | ko |
dc.date.accessioned | 2018-06-16T07:37:20Z | - |
dc.date.available | 2018-06-16T07:37:20Z | - |
dc.date.created | 2018-06-11 | - |
dc.date.created | 2018-06-11 | - |
dc.date.created | 2018-06-11 | - |
dc.date.created | 2018-06-11 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, v.46, no.9, pp.4689 - 4698 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10203/242541 | - |
dc.description.abstract | RPA is known to stimulate the helicase activity of Werner syndrome protein (WRN), but the exact stimulation-mechanism is not understood. We use single-molecule FRET and magnetic tweezers to investigate the helicase activity of WRN and its stimulation by RPA. We show that WRN alone is a weak helicase which repetitively unwind just a few tens of base pairs, but that binding of multiple RPAs to the enzyme converts WRN into a superhelicase that unidirectionally unwinds double-stranded DNA more than 1 kb. Our study provides a good case in which the activity and biological functions of the enzyme may be fundamentally altered by the binding of cofactors. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Multiple RPAs make WRN syndrome protein a superhelicase | - |
dc.type | Article | - |
dc.identifier.wosid | 000433056900033 | - |
dc.identifier.scopusid | 2-s2.0-85061700853 | - |
dc.type.rims | ART | - |
dc.citation.volume | 46 | - |
dc.citation.issue | 9 | - |
dc.citation.beginningpage | 4689 | - |
dc.citation.endingpage | 4698 | - |
dc.citation.publicationname | NUCLEIC ACIDS RESEARCH | - |
dc.identifier.doi | 10.1093/nar/gky272 | - |
dc.contributor.localauthor | Kim, Jaehoon | - |
dc.contributor.nonIdAuthor | Lee, Mina | - |
dc.contributor.nonIdAuthor | Shin, Soochul | - |
dc.contributor.nonIdAuthor | Uhm, Heesoo | - |
dc.contributor.nonIdAuthor | Hong, Heesun | - |
dc.contributor.nonIdAuthor | Kirk, Jaewon | - |
dc.contributor.nonIdAuthor | Kulikowicz, Tomasz | - |
dc.contributor.nonIdAuthor | Ahn, Byungchan | - |
dc.contributor.nonIdAuthor | Bohr, Vilhelm A. | - |
dc.contributor.nonIdAuthor | Hohng, Sungchul | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | WERNER-SYNDROME PROTEIN | - |
dc.subject.keywordPlus | DNA END-RESECTION | - |
dc.subject.keywordPlus | BLOOM-SYNDROME HELICASES | - |
dc.subject.keywordPlus | SINGLE-STRANDED-DNA | - |
dc.subject.keywordPlus | RECQ HELICASES | - |
dc.subject.keywordPlus | MAGNETIC TWEEZERS | - |
dc.subject.keywordPlus | LARGE SUBUNIT | - |
dc.subject.keywordPlus | BLM HELICASE | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | MOLECULE | - |
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