High-level production of trans-cinnamic acid by fed-batch cultivation of Escherichia coli

Cited 8 time in webofscience Cited 0 time in scopus
  • Hit : 249
  • Download : 0
Trans-cinnamic acid is a phenylpropanoid that is widely used in cosmetics, anti-bacterial compounds, anticancer, and flavoring agents. Previously, we succeeded in the generation of L-phenylalanine-high producing Escherichia coli strain by metabolic engineering of the L-phenylalanine biosynthesis pathway. Using this engineered strain, in this study, we developed an E. coli platform for the enhanced production of trans-cinnamic acid that can be generated from L-phenylalanine by phenylalanine ammonia-lyase (PAL)-mediated deamination reaction. To increase the production titer of trans-cinnamic acid, three different promoters and four different nutrient solutions were examined and, using the optimized system (gene expression under Trc promoter and supplementation of casamino acid), we achieved the production of trans-cinnamic acid as high as 697 mgL(-1) in shake flask cultivation. Finally, pH-stat fed-batch fermentations were performed in a lab-scale (2 L) bioreactor with three different feeding solutions (glucose, yeast extract, and casamino acid). When casamino acid was supplied as feeding solution, the production of trans-cinnamic acid as high as 6.9 gL(-1) was achieved.
Publisher
ELSEVIER SCI LTD
Issue Date
2018-05
Language
English
Article Type
Article
Keywords

CELL-DENSITY CULTURE; SACCHAROMYCES-CEREVISIAE; SECRETORY PRODUCTION; CARBON-SOURCES; SUCCINIC ACID; BIOSYNTHESIS; EXPRESSION; FERMENTATION; DERIVATIVES; PATHWAY

Citation

PROCESS BIOCHEMISTRY, v.68, pp.30 - 36

ISSN
1359-5113
DOI
10.1016/j.procbio.2018.01.026
URI
http://hdl.handle.net/10203/242462
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 8 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0