Functional analysis of BC200 RNA in cancer cells.

Abstract

Noncoding DNA consists of 98% of whole human genome and more than 60% of them are consistently transcribed. Noncoding RNAs from that sequences delicately regulate gene expressions in cells. BC200 RNA is a noncoding RNA expressed in neuron cells where it controls local translational initiations. The BC200 RNA is not expressed in normal cells except for neurons, but it is abnormally expressed at several cancer cells. In this study, we analysed the functions of the BC200 RNA in cancer cells. The BC200 RNA promotes cellular motility by stabilizing S100A11 transcripts, which is highly related to cellular motility. We found that when BC200 knockdown were performed, HeLa cell motility was reduced and 29 gene-expressions were altered including the S100A11. We also observed that BC200 RNA was localized at p-bodies. The BC200 RNA seems to regulate specific genes in these granules. Together, our results show that BC200 RNA contributes to cancer developments by specifically regulating cancer-related genes in particular area.