DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Jones, Walton D. | - |
dc.contributor.advisor | 존스, 월튼 | - |
dc.contributor.author | Bahk, Suhyoung | - |
dc.contributor.author | 박서영 | - |
dc.date.accessioned | 2018-05-23T19:34:39Z | - |
dc.date.available | 2018-05-23T19:34:39Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=675722&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/241843 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2017.2,[iv, 72 p. :] | - |
dc.description.abstract | Like most animals, insects rely on olfaction to find food and mates and avoid noxious chemicals and predators. Most insect olfactory sensory neurons (OSNs) express an odorant-specific odorant receptor (OR) along with Orco, the olfactory co-receptor. Orco binds ORs and permits their trafficking to the dendrites of antennal OSNs, where together, they are suggested to form heteromeric, ligand-gated, non-selective cation channels. Within Orco's second intracellular loop, there is an especially well-conserved sequence strongly predicted to be a calmodulin (CaM) binding site (CBS). In this study, I explored the relationship between Orco and CaM \emph{in vivo} in the olfactory neurons of Drosophila melanogaster. I first found OSN-specific knock-down of CaM at the onset of OSN development disrupts the spontaneous firing of OSNs and reduces Orco trafficking to the ciliated dendrites of OSNs. I then generated a series of Orco CBS mutant proteins and found that none of them rescue the Orco-null $Orco^1$ mutant phenotype, which is characterized by an OR protein trafficking defect that blocks spontaneous and odorant-evoked OSN activity. In contrast to an identically constructed wild-type form of Orco that does rescue the $Orco^1$ phenotype, all the Orco CBS mutants remain stuck in the OSN soma. Last, I found CaM's modulation of OR trafficking is activity-dependent. Knock-down of CaM in OSNs after OR expression is well-established has little effect on olfactory responsiveness alone. When combined with an extended exposure to odorant, however, this late-onset CaM knock-down significantly reduces both olfactory sensitivity and the trafficking of Orco only to the ciliated dendrites of OSNs that respond to the exposed odorant. Therefore, I propose a model in which the CaM/Orco interaction allows insect OSNs to maintain appropriate dendritic levels of OR regardless of environmental odorant concentrations. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Insect olfaction | - |
dc.subject | Drosophila melanogaster | - |
dc.subject | odorant receptor | - |
dc.subject | Orco | - |
dc.subject | calmodulin | - |
dc.subject | 곤충 후각 | - |
dc.subject | 초파리 | - |
dc.subject | 후각수용체 | - |
dc.subject | 후각보조수용체 | - |
dc.subject | 칼모듈린 | - |
dc.title | Characterization of calmodulin's regulatory role in insect odorant receptor trafficking and olfactory response | - |
dc.title.alternative | 칼모듈린의 곤충 후각수용체 수송 및 후각반응 제어 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.