Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

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In this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH similar to 5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn to find enzyme mimicking ACs by sol-gel ambient conditions. Through the screening of catalytic activities by the typical colorimetric reaction employing 2,2 0-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) as a substrate with or without H2O2, Fe, Cu, and CoACs are found to exhibit peroxidase-like activity, as well as oxidase-like activity was observed from Ce and MnACs. Among them, CoAC shows exceptionally high peroxidase-like activity, presumably due to its ability to induce electron transfer between substrates and H2O2. CoAC is then used to catalyze the oxidation of Amplex (R) UltraRed (AUR) into a fluorescent end product, which enables a sensitive fluorescent detection of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy is developed, based on enzyme cascade reaction between glucose oxidase (GOx) and CoAC. Using this strategy, a highly linear fluorescence enhancement is verified when the concentration of glucose is increased in a wide range from 10 mu M to 1 mM with a lower detection limit of 5 mu M. The practical diagnostic capability of the assay system is also verified by its use to detect glucose in human blood serum. Based on these results, it is anticipated that CoAC can serve as potent peroxidase mimetics for the detection of clinically important target molecules.
Publisher
MDPI
Issue Date
2018-02
Language
English
Article Type
Article
Citation

SENSORS, v.18, no.2

ISSN
1424-8220
DOI
10.3390/s18020457
URI
http://hdl.handle.net/10203/241591
Appears in Collection
CBE-Journal Papers(저널논문)
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