DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, Yuchao | ko |
dc.contributor.author | Lee, Minji | ko |
dc.contributor.author | Kim, Nury | ko |
dc.contributor.author | Wu, Guoyu | ko |
dc.contributor.author | Deng, Difan | ko |
dc.contributor.author | Kim, Jin Man | ko |
dc.contributor.author | Liu, Xuedong | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Zi, Zhike | ko |
dc.date.accessioned | 2018-03-23T00:16:05Z | - |
dc.date.available | 2018-03-23T00:16:05Z | - |
dc.date.created | 2018-03-20 | - |
dc.date.created | 2018-03-20 | - |
dc.date.created | 2018-03-20 | - |
dc.date.created | 2018-03-20 | - |
dc.date.issued | 2018-02 | - |
dc.identifier.citation | ACS SYNTHETIC BIOLOGY, v.7, no.2, pp.443 - 451 | - |
dc.identifier.issn | 2161-5063 | - |
dc.identifier.uri | http://hdl.handle.net/10203/240950 | - |
dc.description.abstract | Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-beta) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-beta signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-beta signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-beta signaling in time and space. Using the optoTGFBRs system, we show that TGF-beta signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-beta receptor and Smad2 proteins, we characterized the dynamics of TGF-beta signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-beta signaling at the single cell level. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Spatiotemporal Control of TGF-beta Signaling with Light | - |
dc.type | Article | - |
dc.identifier.wosid | 000426012600017 | - |
dc.identifier.scopusid | 2-s2.0-85042185959 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 443 | - |
dc.citation.endingpage | 451 | - |
dc.citation.publicationname | ACS SYNTHETIC BIOLOGY | - |
dc.identifier.doi | 10.1021/acssynbio.7b00225 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Li, Yuchao | - |
dc.contributor.nonIdAuthor | Kim, Nury | - |
dc.contributor.nonIdAuthor | Wu, Guoyu | - |
dc.contributor.nonIdAuthor | Deng, Difan | - |
dc.contributor.nonIdAuthor | Kim, Jin Man | - |
dc.contributor.nonIdAuthor | Liu, Xuedong | - |
dc.contributor.nonIdAuthor | Zi, Zhike | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | cell signaling | - |
dc.subject.keywordAuthor | TGF-beta | - |
dc.subject.keywordAuthor | optogenetics | - |
dc.subject.keywordAuthor | Smad2 | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | OPTOGENETIC CONTROL | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | KINASE-ACTIVITY | - |
dc.subject.keywordPlus | BLUE-LIGHT | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | PATHWAY | - |
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