DC Field | Value | Language |
---|---|---|
dc.contributor.author | Uhm, Heesoo | ko |
dc.contributor.author | Kang, Wooyoung | ko |
dc.contributor.author | Ha, Kook Sun | ko |
dc.contributor.author | Kang, Changwon | ko |
dc.contributor.author | Hohng, Sungchul | ko |
dc.date.accessioned | 2018-02-21T05:35:06Z | - |
dc.date.available | 2018-02-21T05:35:06Z | - |
dc.date.created | 2018-01-29 | - |
dc.date.created | 2018-01-29 | - |
dc.date.created | 2018-01-29 | - |
dc.date.created | 2018-01-29 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.115, no.2, pp.331 - 336 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/240104 | - |
dc.description.abstract | Because RNAs fold as they are being synthesized, their transcription rate can affect their folding. Here, we report the results of single-molecule fluorescence studies that characterize the ligand-dependent cotranscriptional folding of the Escherichia coli thiM riboswitch that regulates translation. We found that the riboswitch aptamer folds into the "off" conformation independent of its ligand, but switches to the "on" conformation during transcriptional pausing near the translational start codon. Ligand binding maintains the riboswitch in the off conformation during transcriptional pauses. We expect our assay will permit the controlled study of the two main physical mechanisms that regulate cotranscriptional folding: transcriptional pausing and transcriptional speed. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.title | Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch | - |
dc.type | Article | - |
dc.identifier.wosid | 000419686400050 | - |
dc.identifier.scopusid | 2-s2.0-85040239343 | - |
dc.type.rims | ART | - |
dc.citation.volume | 115 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 331 | - |
dc.citation.endingpage | 336 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.1712983115 | - |
dc.contributor.localauthor | Kang, Changwon | - |
dc.contributor.nonIdAuthor | Uhm, Heesoo | - |
dc.contributor.nonIdAuthor | Kang, Wooyoung | - |
dc.contributor.nonIdAuthor | Ha, Kook Sun | - |
dc.contributor.nonIdAuthor | Hohng, Sungchul | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | thiamine pyrophosphate riboswitch | - |
dc.subject.keywordAuthor | single-molecule FRET | - |
dc.subject.keywordAuthor | cotranscriptional folding | - |
dc.subject.keywordPlus | THI-BOX RIBOSWITCH | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | TPP RIBOSWITCH | - |
dc.subject.keywordPlus | RNA-POLYMERASE | - |
dc.subject.keywordPlus | LIGAND RECOGNITION | - |
dc.subject.keywordPlus | ENERGY LANDSCAPE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | APTAMER | - |
dc.subject.keywordPlus | REVEAL | - |
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