This paper proposed a high gain spherical dielectric resonator antenna (DRA) operating on higher-order mode excited by microstrip patch. A microstrip patch was used to excite TEn01 mode on a dielectric sphere. The excited dielectric sphere operates as higher-order mode spherical DRA with high gain. Impedance matching method for conventional microstrip patch could be applied to the proposed antenna. Size minimization method for the excitation microstrip patch was also described on this paper. A prototype antenna operating on TE301 mode at 5.8 GHz was fabricated by a ceramic dielectric material, which dielectric constant is 13, and showed the peak gain of 9.03 dBi.