Xylitol Producing Microorganism Introduced with Arabinose Metabolic Pathway and Production Method of Xylitol Using the Same아라비노스 대사경로가 도입된 자일리톨 생산균주 및 이를 이용한자일리톨생산방법

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The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose/arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in Candida tropicalis, codon optimization was performed. Then, each gene was inserted in the gene expression cassette containing the glyceraldehyde-3-phosphate dehydrogenase promoter and the selection marker URA3, which was introduced into Candida sp. microorganism. As a result, arabitol, the byproduct interrupting the purification and crystallization of xylitol could be inhibited, making the production method of xylitol of the present invention more efficient. The xylitol producing microorganism introduced with arabinose metabolic pathway of the present invention can be effectively used for the production of xylitol with high productivity by inhibiting the generation of arabitol.
Assignee
KAIST
Country
US (United States)
Issue Date
2013-01-01
Application Date
2011-01-18
Application Number
13391073
Registration Date
2013-01-01
Registration Number
8,343,736
URI
http://hdl.handle.net/10203/234531
Appears in Collection
BS-Patent(특허)
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