Mass spectrometric methods for sequencing nucleic acids

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A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.
Assignee
Sequenom, Inc.
Country
US (United States)
Issue Date
2001-07-31
Application Date
1997-12-15
Application Number
08990851
Registration Date
2001-07-31
Registration Number
06268131
URI
http://hdl.handle.net/10203/233830
Appears in Collection
BS-Patent(특허)
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