Method for forming a reversible protein nanocluster using light in a cell셀에서 가역적 단백질 나노 집단 사용하는 광을 형성하기 위한 방법

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To efficiently analyze interaction and function between proteins, the present invention relates to a method for forming a light-induced protein nanocluster, comprising: an expression vector preparation step of preparing a first expression vector including polynucleotides coding a first fusion protein including a light-induced heterodimer-forming protein and a first self-assembly protein, and a second expression vector including polynucleotides coding a couple protein that forms a homodimer with said light-induced heterodimer-forming protein, or a second fusion protein including said couple protein and a second self-assembly protein; a transformed cell, tissue or individual preparation step of transforming cells, tissues or individuals using said first expression vector and second expression vector; and a light radiation step of radiating light having a wavelength for inducing the formation of heterodimer between said light-induced heterodimer-forming protein and said couple protein, to said transformed cells, tissue or individuals.
Assignee
KAIST
Country
US (United States)
Issue Date
2017-07-18
Application Date
2012-09-28
Application Number
14348757
Registration Date
2017-07-18
Registration Number
9708381
URI
http://hdl.handle.net/10203/229451
Appears in Collection
BS-Patent(특허)
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