Intracellular Protein Delivery System Using a Target-Specific Repebody and Translocation Domain of Bacterial Exotoxin

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dc.contributor.authorKim, Hee Yeonko
dc.contributor.authorKang, Jung Aeko
dc.contributor.authorRyou, Jeong-Hyunko
dc.contributor.authorLee, Gyeong Heeko
dc.contributor.authorChoi, Dae Seongko
dc.contributor.authorLee, Dong Eunko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2017-12-19T03:02:00Z-
dc.date.available2017-12-19T03:02:00Z-
dc.date.created2017-12-11-
dc.date.created2017-12-11-
dc.date.issued2017-11-
dc.identifier.citationACS CHEMICAL BIOLOGY, v.12, no.11, pp.2891 - 2897-
dc.identifier.issn1554-8929-
dc.identifier.urihttp://hdl.handle.net/10203/228590-
dc.description.abstractWith the high efficacy of protein-based therapeutics and plenty of intracellular drug targets, cytosolic protein delivery in a cell-specific manner has attracted considerable attention in the field of precision medicine. Herein, we present an intracellular protein delivery system based on a target-specific repebody and the translocation domain of Pseudomonas aeruginosa exotoxin A. The delivery platform was constructed by genetically fusing an EGFR-specific repebody as a targeting moiety to the translocation domain, while a protein cargo was fused to the C-terminal end of the delivery platform. The delivery platform was revealed to efficiently translocate a protein cargo to the cytosol in a target-specific manner. We demonstrate the utility and potential of the delivery platform by showing a remarkable tumor regression with negligible toxicity in a xenograft mice model when gelonin was used as the cytotoxic protein cargo. The present platform can find wide applications to the cell-selective cytosolic delivery of diverse proteins in many areas.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.subjectIN-VITRO-
dc.subjectDRUG-DELIVERY-
dc.subjectCANCER-
dc.subjectTOXIN-
dc.subjectVIVO-
dc.subjectEGFR-
dc.subjectANTIBODY-
dc.subjectTHERAPY-
dc.subjectGELONIN-
dc.subjectDESIGN-
dc.titleIntracellular Protein Delivery System Using a Target-Specific Repebody and Translocation Domain of Bacterial Exotoxin-
dc.typeArticle-
dc.identifier.wosid000416204500024-
dc.identifier.scopusid2-s2.0-85034630339-
dc.type.rimsART-
dc.citation.volume12-
dc.citation.issue11-
dc.citation.beginningpage2891-
dc.citation.endingpage2897-
dc.citation.publicationnameACS CHEMICAL BIOLOGY-
dc.identifier.doi10.1021/acschembio.7b00562-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.nonIdAuthorKang, Jung Ae-
dc.contributor.nonIdAuthorLee, Gyeong Hee-
dc.contributor.nonIdAuthorChoi, Dae Seong-
dc.contributor.nonIdAuthorLee, Dong Eun-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusIN-VITRO-
dc.subject.keywordPlusDRUG-DELIVERY-
dc.subject.keywordPlusCANCER-
dc.subject.keywordPlusTOXIN-
dc.subject.keywordPlusVIVO-
dc.subject.keywordPlusEGFR-
dc.subject.keywordPlusANTIBODY-
dc.subject.keywordPlusTHERAPY-
dc.subject.keywordPlusGELONIN-
dc.subject.keywordPlusDESIGN-
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