Current status and perspectives of genome editing technology for microalgae

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Genome editing techniques are critical for manipulating genes not only to investigate their functions in biology but also to improve traits for genetic engineering in biotechnology. Genome editing has been greatly facilitated by engineered nucleases, dubbed molecular scissors, including zinc-finger nuclease (ZFN), TAL effector endonuclease (TALEN) and clustered regularly interspaced palindromic sequences (CRISPR)/Cas9. In particular, CRISPR/Cas9 has revolutionized genome editing fields with its simplicity, efficiency and accuracy compared to previous nucleases. CRISPR/Cas9-induced genome editing is being used in numerous organisms including microalgae. Microalgae have been subjected to extensive genetic and biological engineering due to their great potential as sustainable biofuel and chemical feedstocks. However, progress in microalgal engineering is slow mainly due to a lack of a proper transformation toolbox, and the same problem also applies to genome editing techniques. Given these problems, there are a few reports on successful genome editing in microalgae. It is, thus, time to consider the problems and solutions of genome editing in microalgae as well as further applications of this exciting technology for other scientific and engineering purposes.
Publisher
BIOMED CENTRAL LTD
Issue Date
2017-11
Language
English
Article Type
Review
Keywords

ZINC-FINGER NUCLEASES; DOMINANT SELECTABLE MARKER; AGROBACTERIUM-MEDIATED TRANSFORMATION; DIATOM PHAEODACTYLUM-TRICORNUTUM; ALGA CHLAMYDOMONAS-REINHARDTII; HIGH-VELOCITY MICROPROJECTILES; NITRATE REDUCTASE GENE; CRISPR-CAS SYSTEM; GREEN-ALGA; KNOCK-IN

Citation

BIOTECHNOLOGY FOR BIOFUELS, v.10

ISSN
1754-6834
DOI
10.1186/s13068-017-0957-z
URI
http://hdl.handle.net/10203/228448
Appears in Collection
CBE-Journal Papers(저널논문)
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