DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ahn, Soyeon | ko |
dc.contributor.author | Choe, Kibaek | ko |
dc.contributor.author | Lee, Seunghun | ko |
dc.contributor.author | Kim, Kangsan | ko |
dc.contributor.author | Song, Eunjoo | ko |
dc.contributor.author | Seo, Howon | ko |
dc.contributor.author | Kim, In-June | ko |
dc.contributor.author | Kim, Pilhan | ko |
dc.date.accessioned | 2017-12-19T00:58:11Z | - |
dc.date.available | 2017-12-19T00:58:11Z | - |
dc.date.created | 2017-11-28 | - |
dc.date.created | 2017-11-28 | - |
dc.date.created | 2017-11-28 | - |
dc.date.created | 2017-11-28 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | PLOS ONE, v.12, no.11 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/228440 | - |
dc.description.abstract | Bone marrow is a vital tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. Bone marrow transplantation (BMT) has been widely performed in patients with blood disorders and cancers. However, the cellular-level behaviors of the transplanted bone marrow cells over wide-areas of the host bone marrow after the BMT are not fully understood yet. In this work, we performed a longitudinal wide-area cellular-level observation of the calvarial bone marrow after the BMT in vivo. Using a H2B-GFP/beta-actin-DsRed double-transgenic mouse model as a donor, a subcellular-level nuclear-cytoplasmic visualization of the transplanted bone marrow cells was achieved, which enabled a direct in vivo dynamic monitoring of the distribution and proliferation of the transplanted bone marrow cells. The same spots in the wide-area of the calvarial bone marrow were repeatedly identified using fluorescently labeled vasculature as a distinct landmark. It revealed various dynamic cellular-level behaviors of the transplanted BM cells in early stage such as cluster formation, migration, and active proliferation in vivo. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Intravital longitudinal wide-area imaging of dynamic bone marrow engraftment and multilineage differentiation through nuclear-cytoplasmic labeling | - |
dc.type | Article | - |
dc.identifier.wosid | 000414377900036 | - |
dc.identifier.scopusid | 2-s2.0-85032824410 | - |
dc.type.rims | ART | - |
dc.citation.volume | 12 | - |
dc.citation.issue | 11 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0187660 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, In-June | - |
dc.contributor.localauthor | Kim, Pilhan | - |
dc.contributor.nonIdAuthor | Kim, Kangsan | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | HEMATOPOIETIC STEM-CELLS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | CONFOCAL MICROSCOPY | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | MULTIPHOTON MICROSCOPY | - |
dc.subject.keywordPlus | FLUORESCENT PROTEINS | - |
dc.subject.keywordPlus | NICHE | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | TRACKING | - |
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