DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Chang Yeol | ko |
dc.contributor.author | Kim, Hyo Yong | ko |
dc.contributor.author | Ahn, Jun Ki | ko |
dc.contributor.author | Park, Ki Soo | ko |
dc.contributor.author | Park, Hyun Gyu | ko |
dc.date.accessioned | 2017-11-08T02:23:48Z | - |
dc.date.available | 2017-11-08T02:23:48Z | - |
dc.date.created | 2017-10-23 | - |
dc.date.created | 2017-10-23 | - |
dc.date.issued | 2017-10 | - |
dc.identifier.citation | RSC ADVANCES, v.7, no.74, pp.47143 - 47147 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://hdl.handle.net/10203/226742 | - |
dc.description.abstract | We herein describe a rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification (eSDA). The developed strategy utilizes specially designed DNA probes that form mismatched thymine-thymine (T-T) base pairs at their 30-ends. In the absence of Hg2+, the mismatched T-T base pairs prohibit the DNA polymerase-mediated extension and the subsequent eSDA does not occur. On the other hand, the presence of Hg2+ that mediates the formation of stable T-Hg2+-T base pairs, enables the extension of DNA probes by DNA polymerase. As a result, a large number of duplexes are produced through the eSDA, which is simply monitored in real-time by the gradual fluorescence enhancement from SYBR green I. By employing this strategy, we detected Hg2+ down to 2.95 pM within 30 min, and the practical applicability of this method was successfully demonstrated by detecting Hg2+ in tap water. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | FLUORESCENCE DETECTION | - |
dc.subject | SELECTIVE DETECTION | - |
dc.subject | POLYMERASE-ACTIVITY | - |
dc.subject | GOLD NANOPARTICLES | - |
dc.subject | DNA AMPLIFICATION | - |
dc.subject | MERCURY | - |
dc.subject | IONS | - |
dc.subject | SENSOR | - |
dc.subject | HEALTH | - |
dc.subject | ASSAY | - |
dc.title | Rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification | - |
dc.type | Article | - |
dc.identifier.wosid | 000412451500071 | - |
dc.identifier.scopusid | 2-s2.0-85030832692 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 74 | - |
dc.citation.beginningpage | 47143 | - |
dc.citation.endingpage | 47147 | - |
dc.citation.publicationname | RSC ADVANCES | - |
dc.identifier.doi | 10.1039/c7ra09226a | - |
dc.contributor.localauthor | Park, Hyun Gyu | - |
dc.contributor.nonIdAuthor | Park, Ki Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FLUORESCENCE DETECTION | - |
dc.subject.keywordPlus | SELECTIVE DETECTION | - |
dc.subject.keywordPlus | POLYMERASE-ACTIVITY | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | DNA AMPLIFICATION | - |
dc.subject.keywordPlus | MERCURY | - |
dc.subject.keywordPlus | IONS | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | ASSAY | - |
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