Rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification

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We herein describe a rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification (eSDA). The developed strategy utilizes specially designed DNA probes that form mismatched thymine-thymine (T-T) base pairs at their 30-ends. In the absence of Hg2+, the mismatched T-T base pairs prohibit the DNA polymerase-mediated extension and the subsequent eSDA does not occur. On the other hand, the presence of Hg2+ that mediates the formation of stable T-Hg2+-T base pairs, enables the extension of DNA probes by DNA polymerase. As a result, a large number of duplexes are produced through the eSDA, which is simply monitored in real-time by the gradual fluorescence enhancement from SYBR green I. By employing this strategy, we detected Hg2+ down to 2.95 pM within 30 min, and the practical applicability of this method was successfully demonstrated by detecting Hg2+ in tap water.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2017-10
Language
English
Article Type
Article
Keywords

FLUORESCENCE DETECTION; SELECTIVE DETECTION; POLYMERASE-ACTIVITY; GOLD NANOPARTICLES; DNA AMPLIFICATION; MERCURY; IONS; SENSOR; HEALTH; ASSAY

Citation

RSC ADVANCES, v.7, no.74, pp.47143 - 47147

ISSN
2046-2069
DOI
10.1039/c7ra09226a
URI
http://hdl.handle.net/10203/226742
Appears in Collection
CBE-Journal Papers(저널논문)
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