Desulfurization of dibenzothiophene and diesel oil by metabolically engineered Escherichia coli

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The desulfurization genes (dszABC) were cloned from Gordonia nitida. Nucleotide sequences similarity between the dszABC genes of G. nitida and those of Rhodococcus rhodochrous IGTS8 was 89%. The similarities of deduced amino acids between the two were 86% for DszA, 86% for DszB, and 90% for DszC. The G. nitida dszABC genes were expressed in several different Escherichia coli strains under an inducible trc promoter. Cultivation of these metabolically engineered E. coli strains in the presence of 0.2mM dibenzothiophene (DBT) allowed the conversion of DBT to 2-hydroxybiphenyl (2-HBP), which is the final metabolite of the sulfur-specific desulfurization pathway. The maximum conversion of DBT to 2-HBP was 16% in 60h. Recombinant E. coli was applied for the deep desulfurization of diesel oil supplemented into the medium at 15% of sulfur/l to 212.5 mg sulfur/l, resulting in the removal of 15% of sulfur in diesel oil in 60h.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2003-08
Language
English
Article Type
Article
Keywords

SP STRAIN IGTS8; BIODESULFURIZATION; GENES; CULTURE

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.13, pp.578 - 583

ISSN
1017-7825
URI
http://hdl.handle.net/10203/22627
Appears in Collection
CBE-Journal Papers(저널논문)
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