RNA activation-independent DNA targeting of the Type III CRISPR-Cas system by a Csm complex

Cited 10 time in webofscience Cited 0 time in scopus
  • Hit : 394
  • Download : 0
The CRISPR-Cas system is an adaptive and heritable immune response that destroys invading foreign nucleic acids. The effector complex of the Type III CRISPR-Cas system targets RNA and DNA in a transcription-coupled manner, but the exact mechanism of DNA targeting by this complex remains elusive. In this study, an effector Csm holocomplex derived from Thermococcus onnurineus is reconstituted with a minimalistic combination of Csm1(1)2(1)3(3)4(1)5(1), and shows RNA targeting and RNA-activated single-stranded DNA (ssDNA) targeting activities. Unexpectedly, in the absence of an RNA transcript, it cleaves ssDNA containing a sequence complementary to the bound crRNA guide region in a manner dependent on the HD domain of the Csm1 subunit. This nuclease activity is blocked by a repeat tag found in the host CRISPR loci. The specific cleavage of ssDNA without a target RNA suggests a novel ssDNA targeting mechanism of the Type III system, which could facilitate the efficient and complete degradation of foreign nucleic acids.
Publisher
WILEY
Issue Date
2017-05
Language
English
Article Type
Article
Keywords

CRYSTAL-STRUCTURE; EFFECTOR COMPLEX; STRUCTURAL BASIS; IMMUNE-SYSTEM; CMR COMPLEX; CLEAVAGE; RECOGNITION; CASCADE; INTERFERENCE; PROKARYOTES

Citation

EMBO REPORTS, v.18, no.5, pp.826 - 840

ISSN
1469-221X
DOI
10.15252/embr.201643700
URI
http://hdl.handle.net/10203/223910
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 10 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0